Rabbit Polyclonal AKT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKT3 aa 1-200.
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- Phytotherapy research : PTR 37:4149-4165202311-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E /ERβ signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lyu,Xiaojia Wei,Xue Yang,Jiayuan Li,Guohui Wan,Yuqing Wang,Zhuangzhuang Hao,Yifan Lu,Jianyou Guo,Jinli ShiPubMed 37300355
- Aging 14:8046-80602022Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanchao Hu,Yajie Fan,Chunyan Zhang,Congxia WangPubMed 36227173
- Oncology letters 23:572022LINC01207 promotes prostate cancer progression by sponging miR-1182 to upregulate AKT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaming Qin,Cheng Ni,Biyong Tan,Shengfei Huang,Bingqing Deng,Zhihua HuangPubMed 34992689
- Thoracic cancer 12:3223-32352021Circ_0016760 accelerates non-small-cell lung cancer progression through miR-646/AKT3 signaling in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Chen,Long Zhou,Ruizhi Ran,Jinqi Huang,Yong Zheng,Maohui Xing,Yanli CaiPubMed 34658165
- Cancer biology & medicine :2021Akt isoforms differentially provide for chemoresistance in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Ma,Hanshuang Shao,Xia Jiang,Zhou Wang,Chuanyue Cary Wu,Diana Whaley,Alan WellsPubMed 34591413
- Oncology reports 46:2021Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zuo,Weiling Li,Xiaofang Yan,Tieliang Ma,Yan Ren,Meijuan Hua,Huiyun Yang,Haifeng Wu,Hongdi ZhuPubMed 34278482
- Journal of the European Academy of Dermatology and Venereology : JEADV 35:203-2102020Increased expression profile of NCSTN, Notch and PI3K/AKT3 in hidradenitis suppurativa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Hessam,T Gambichler,M Skrygan,L Scholl,M Sand,T Meyer,E Stockfleth,F G BecharaPubMed 32978818
- Nature communications 11:48752020Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianlin Liu et. al.PubMed 32978388
- The journal of gene medicine 22:e32692020Circ_0000144 facilitates the progression of thyroid cancer via the miR-217/AKT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Xiang Fan,Huan-Yang Shi,Yu-Lin Hu,Xiao-Li JinPubMed 32890417
- Oncology reports 44:1282-12922020MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Wang et. al.PubMed 32705237
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AKT3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y243
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Alternative names
PKBG, AKT3, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, RAC-PK-gamma, STK-2, PKB gamma
Recommended products
Rabbit Polyclonal AKT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKT3 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AKT3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y243
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AKT3 also known as protein kinase B gamma plays an important role in various cellular processes. This serine/threonine kinase with a molecular mass of approximately 56 kDa functions as part of the AKT family which includes AKT1 and AKT2. AKT3 expresses highly in the brain tissues with significant expression also observed in the heart and kidneys. As an isoform of the AKT kinase it participates in signaling pathways essential for cell survival and metabolism.
- Biological function summary
AKT3 participates in regulating cell proliferation and survival. It operates as part of the PI3K/AKT signaling complex a central hub for signaling growth factors. This pathway is critical for transmitting signals from the cell surface to the nucleus affecting transcription and cell cycle progression. AKT3 protein specifically contributes significant roles in neuronal development and function due to its high expression within the nervous system.
- Pathways
AKT3 influences the PI3K/AKT and mTOR pathways. These pathways regulate cell growth metabolism and survival playing significant roles in normal cellular functions and cancer. AKT3 interacts closely with other signaling molecules including mTORC1 and mTORC2 complexes. While participating in these pathways AKT3 coordinates with the protein E17K which is pattern related with alterations leading to abnormal signaling and growth.
- Associated diseases and disorders
AKT3 is implicated in cancer and neurological disorders. Aberrations in its signaling can lead to tumorigenesis as seen in glioblastomas. Abnormal AKT3 activity associates with epilepsy and other neurodevelopmental disorders emphasizing its impact on neuronal functions. Connecting AKT3 with disorders the protein E17K mutation in AKT3 dysregulates normal kinase activity contributing to these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-AKT3 antibody (ab152157)
All lanes: Western blot - Anti-AKT3 antibody (ab152157) at 1/1000 dilution
Lane 1: Non-transfected 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody (ab152157)
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue, staining AKT3 using ab152157 at a 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-AKT3 antibody (ab152157)
Immunofluorescent analysis of methanol fixed MCF-7 cells labeling AKT3 with ab152157 at 1 in 500 dilution.
Blue: Hoechst 33342 staining.
Western blot - Anti-AKT3 antibody (ab152157)
10% SDS PAGE
All lanes: Western blot - Anti-AKT3 antibody (ab152157) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 56 kDa
Western blot - Anti-AKT3 antibody (ab152157)
7.5% SDS PAGE
All lanes: Western blot - Anti-AKT3 antibody (ab152157) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 56 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-AKT3 antibody (ab152157)
AKT3 western blot using anti-AKT3 antibody ab152157. Publication image and figure legend from Teng, Y., Zhang, Y., et al., 2015, Oncotarget, PubMed 26512921.
ab152157 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab152157 please see the product overview.
miR-29b directly targets and thus negatively regulates AKT2 and AKT3A. A schematic shows the prediction and screening process of miR-29b downstream target gene involved in cancerous glycolysis regulation by a series of microRNA bioinformatics softwares; B. Expression analysis between miR-29b and predicted downstream target genes using the NCI-60 expression profiling data. Among the five selected genes, AKT2 and AKT3 showed a significantly negative correlation against miR-29b level; C. qPCR results indicate that miR-29b inhibition increased AKT2 and AKT3 levels in A2780 cells, and miR-29b overexpression decreased AKT2 and AKT3 levels in SKOV3 cells. While no change in AKT1 levels was observed in both conditions; D. Western blot results show that miR-29b inhibition increased AKT2 and AKT3 levels, and miR-29b overexpression decreased AKT2 and AKT3 levels in both A2780 and SKOV3 cells. While no change in AKT1 levels was observed in both conditions; E. Schematic representation of different AKT2/AKT3 3′UTR luciferase reporters used in the transfection experiments are depicted; F. SKOV3/A2780 cells were co-transfected with either miR-29b or control mimics and 200 ng pGL3 reporter construct containing wild type or miR-29b site mutated 3′UTR of AKT2/3. The relative firefly luciferase activities normalized with renilla luciferase were measured 48 hours post-transfection and results are plotted as percentage change over respective controls. G. Immunohistochemical results reveal that expression of AKT2 and AKT3 is higher in EOC epithelia than in normal ovarian epithelia; H. Immunohistochemical results indicate a negative correlation between miR-29b and AKT2/3 levels in EOC epithelia. All IHC images were photographed under 400× magnification. Data are presented as means ± SEM, n = 3. *p < 0.05 versus control.
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