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AB231715

Anti-Alanine Transaminase antibody

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(2 Publications)

Rabbit Polyclonal Alanine Transaminase antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPT aa 100-300.

View Alternative Names

AAT1, GPT1, GPT, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human liver tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Alanine Transaminase using ab231715 at 20 μg/m in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human thryoid cancer tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. AEC staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human liver tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. AEC staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alanine Transaminase antibody (AB231715)

Formalin-fixed, paraffin-embedded human liver tissue stained for Alanine Transaminase using ab231715 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Alanine Transaminase antibody (AB231715)
  • WB

Supplier Data

Western blot - Anti-Alanine Transaminase antibody (AB231715)

All lanes:

Western blot - Anti-Alanine Transaminase antibody (ab231715) at 2 µg/mL

All lanes:

Rat liver lysate

Predicted band size: 55 kDa

false

Western blot - Anti-Alanine Transaminase antibody (AB231715)
  • WB

Supplier Data

Western blot - Anti-Alanine Transaminase antibody (AB231715)

All lanes:

Western blot - Anti-Alanine Transaminase antibody (ab231715) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 55 kDa

false

Western blot - Anti-Alanine Transaminase antibody (AB231715)
  • WB

Supplier Data

Western blot - Anti-Alanine Transaminase antibody (AB231715)

All lanes:

Western blot - Anti-Alanine Transaminase antibody (ab231715) at 2 µg/mL

All lanes:

Recombinant human Alanine Transaminase protein

Predicted band size: 55 kDa

false

Western blot - Anti-Alanine Transaminase antibody (AB231715)
  • WB

Supplier Data

Western blot - Anti-Alanine Transaminase antibody (AB231715)

All lanes:

Western blot - Anti-Alanine Transaminase antibody (ab231715) at 2 µg/mL

All lanes:

Mouse liver lysate

Predicted band size: 55 kDa

false

Western blot - Anti-Alanine Transaminase antibody (AB231715)
  • WB

Supplier Data

Western blot - Anti-Alanine Transaminase antibody (AB231715)

All lanes:

Western blot - Anti-Alanine Transaminase antibody (ab231715) at 2 µg/mL

All lanes:

Mouse kidney lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GPT aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P24298

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231715 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information GPT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:4950-4978 PubMed39358551

2024

SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function.

Applications

Unspecified application

Species

Unspecified reactive species

Gyan Ranjan,Paras Sehgal,Vinod Scaria,Sridhar Sivasubbu

Cancers 12: PubMed32660149

2020

Glutamic-Pyruvic Transaminase 1 Facilitates Alternative Fuels for Hepatocellular Carcinoma Growth-A Small Molecule Inhibitor, Berberine.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Hor-Yue Tan,Sha Li,Ning Wang,Yibin Feng
View all publications
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