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AB34832

Anti-Alanine Transaminase antibody

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(1 Publication)

Sheep Polyclonal Alanine Transaminase antibody. Suitable for ELISA, WB and reacts with Pig samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Pig GPT.

View Alternative Names

AAT1, GPT1, GPT, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Pig

Applications

WB, ELISA

applications

Immunogen

Full Length Protein corresponding to Pig GPT. The exact immunogen used to generate this antibody is proprietary information.

P13191

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Alternative Products

Primary Antibodies

AB202083

Anti-Alanine Transaminase antibody [EPR19616]

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Primary Antibodies

AB137531

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Primary Antibodies

AB236658

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Primary Antibodies

AB231715

Anti-Alanine Transaminase antibody

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Primary Antibodies

AB231629

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Primary Antibodies

AB240363

Anti-Alanine Transaminase antibody [EPR19616] - BSA and Azide free

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Complementary Products

Assay Kits

AB234579

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Assay Kits

AB241035

ALT Assay Kit (384 well, Colorimetric/Fluorometric)

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Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

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Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

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Assay Kits

AB263882

Mouse AST ELISA Kit (Aspartate Aminotransferase)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Purification notes
This product was prepared from monospecific antiserum by a delipidation and defibrination.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information GPT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 39:1509-1529 PubMed35842499

2022

Plk2-mediated phosphorylation and translocalization of Nrf2 activates anti-inflammation through p53/Plk2/p21 signaling in acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Eun Kim,Hye Eun Byeon,Dae-Hoon Kim,Sang Geon Kim,Hyungshin Yim
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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