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Rabbit Recombinant Monoclonal Alanine Transaminase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.


Images

Western blot - Anti-Alanine Transaminase antibody [EPR19616] (AB202083), expandable thumbnail
  • Western blot - Anti-Alanine Transaminase antibody [EPR19616] (AB202083), expandable thumbnail
  • Western blot - Anti-Alanine Transaminase antibody [EPR19616] (AB202083), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (AB202083), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (AB202083), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Species

Rat

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/70

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Alanine Transaminase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19616

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.

Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Associated diseases and disorders

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Blocking/Dilution buffer: 5% NFDM/TBST..

    All lanes: Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 55 kDa

    Observed band size: 55 kDa

    Exposure time: 3min

  • Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 3 and 4: 3 minutes; Lane 5: 4 seconds.

    All lanes: Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1: Human skeletal muscle lysate at 20 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Mouse muscle lysate at 20 µg

    Lane 4: Rat muscle lysate at 20 µg

    Lane 5: Mouse liver lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 55 kDa, 60 kDa

    Observed band size: 55 kDa, 60 kDa

  • Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 30 seconds.

    All lanes: Western blot - Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Rat brain lysate at 10 µg

    Lane 4: Rat heart lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa

    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594 ) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202083 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202083 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Alanine Transaminase antibody [EPR19616] (ab202083), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal- Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody (blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/500 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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