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AB226259

Anti-AlaRS antibody

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(2 Publications)

Rabbit Polyclonal AlaRS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AARS1 aa 250-350.

View Alternative Names

AARS, AARS1, Alanyl-tRNA synthetase, Protein lactyltransferase AARS1, Renal carcinoma antigen NY-REN-42, AlaRS

2 Images
Immunoprecipitation - Anti-AlaRS antibody (AB226259)
  • IP

Supplier Data

Immunoprecipitation - Anti-AlaRS antibody (AB226259)

AlaRS was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226259 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226259 at 0.4 μg/ml.

Lane 1 : ab226259 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-AlaRS antibody (ab226259)

Predicted band size: 107 kDa

false

Western blot - Anti-AlaRS antibody (AB226259)
  • WB

Supplier Data

Western blot - Anti-AlaRS antibody (AB226259)

All lanes:

Western blot - Anti-AlaRS antibody (ab226259) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 107 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human AARS1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P49588

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab226259 was affinity purified using an epitope specific to AlaRS immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AlaRS also known as alanyl-tRNA synthetase is an enzyme critically involved in protein synthesis. It has an approximate molecular mass of 100 kDa. AlaRS catalyzes the attachment of alanine to its corresponding tRNA molecule ensuring the incorporation of alanine into a growing polypeptide chain. This enzymatic activity makes it an important component in the translation machinery. AlaRS shows expression across a wide variety of tissues reflecting its essential role in cellular function and biosynthesis.
Biological function summary

The enzyme plays an essential role without functioning as part of a complex. AlaRS participates in the aminoacylation of tRNA a process ensuring fidelity during protein translation. It charges tRNA with alanine maintaining accuracy in protein synthesis. This process is vital for cellular function because incorrect amino acid incorporation can lead to misfolded proteins and malfunction.

Pathways

AlaRS plays an important part in the translation pathway an essential component of the wider protein biosynthesis network. It interacts closely with ribosomal proteins during translation. AlaRS also links to the aminoacyl-tRNA biosynthesis pathway sharing functional similarities with other aminoacyl-tRNA synthetases such as GlnRS and GlyRS that attach amino acids to their corresponding tRNAs.

AlaRS has connections with Charcot-Marie-Tooth disease and specific types of cancer. Mutations in the gene encoding AlaRS can lead to Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. The enzyme also shows altered expression in some cancers connecting it indirectly with oncogenic proteins that contribute to tumor progression. These associations highlight AlaRS as a target for understanding pathologies related to protein synthesis dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction : alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed : 27622773, PubMed : 27911835, PubMed : 28493438, PubMed : 33909043). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed : 27622773, PubMed : 27911835, PubMed : 28493438, PubMed : 29273753). In presence of high levels of lactate, also acts as a protein lactyltransferase that mediates lactylation of lysine residues in target proteins, such as TEAD1, TP53/p53 and YAP1 (PubMed : 38512451, PubMed : 38653238). Protein lactylation takes place in a two-step reaction : lactate is first activated by ATP to form lactate-AMP and then transferred to lysine residues of target proteins (PubMed : 38512451, PubMed : 38653238). Acts as an inhibitor of TP53/p53 activity by catalyzing lactylation of TP53/p53 (PubMed : 38653238). Acts as a positive regulator of the Hippo pathway by mediating lactylation of TEAD1 and YAP1 (PubMed : 38512451).
See full target information AARS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 21:2024-2033 PubMed39349603

2024

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqi Xu,Linzhen Kong,Jingfei Cheng,Khatoun Al Moussawi,Xiufei Chen,Aleema Iqbal,Peter A C Wing,James M Harris,Senko Tsukuda,Azman Embarc-Buh,Guifeng Wei,Alfredo Castello,Skirmantas Kriaucionis,Jane A McKeating,Xin Lu,Chun-Xiao Song

Human molecular genetics 32:2177-2191 PubMed37010095

2023

A humanized yeast model reveals dominant-negative properties of neuropathy-associated alanyl-tRNA synthetase mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Meyer-Schuman,Sheila Marte,Tyler J Smith,Shawna M E Feely,Marina Kennerson,Garth Nicholson,Mike E Shy,Kristin S Koutmou,Anthony Antonellis
View all publications
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