AB115216

Anti-Alas1 antibody

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(1 Publication)

Rabbit Polyclonal Alas1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ALAS1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ALAS3, ALASH, OK/SW-cl.121, ALAS1, ALAS-H, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, Delta-aminolevulinate synthase 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alas1 antibody (AB115216)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alas1 antibody (AB115216)

Human skin (formalin fixed, paraffin embedded) stained with ab115216 at 5 ug/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ALAS1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P13196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Alas1' also known as 5-Aminolevulinic acid synthase 1 is a mitochondrial enzyme with a mass of approximately 64 kDa. This enzyme plays an important role in heme biosynthesis catalyzing the first step in the pathway by converting glycine and succinyl-CoA to 5-aminolevulinic acid. Unfortunately it does not function alone and often requires the presence of pyridoxal phosphate as a cofactor. 'Alas1' is ubiquitously expressed in a variety of tissues including liver and bone marrow where heme production is critical.

Biological function summary

'Alas1' serves as a rate-limiting enzyme in the heme synthesis pathway. Being the first step it sets the pace for the entire process. It does not form part of any larger protein complex and operates with its required cofactors. Beyond metabolic roles its regulation influences several cellular processes ensuring heme availability precisely matches cellular demand.

Pathways

'Alas1' performs a fundamental role in the mitochondrial heme biosynthesis pathway. This pathway is essential for synthesizing heme a component critical to various cellular functions such as oxygen transport and electron transfer. 'Alas1' interacts with proteins like ferrochelatase the enzyme completing the heme biosynthesis pathway. Coordination between 'Alas1' and other proteins ensures efficient production of heme enabling proper cellular function and adaptation to changes in cellular and systemic conditions.

'Alas1' is involved in conditions such as acute intermittent porphyria and certain anemias. Its dysregulation can lead to impaired heme production with downstream effects on cell metabolism and organ function. 'Alas1' has been shown to interact with HMBS (hydroxymethylbilane synthase) a protein linked to different types of porphyria. Understanding its role in these conditions could lead to targeted therapies that modulate its activity to alleviate symptoms or correct underlying biochemical dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products.

See full target information ALAS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virulence 9:588-603 PubMed29338543

2018

Lucidone suppresses dengue viral replication through the induction of heme oxygenase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chun Chen,Chin-Kai Tseng,Chun-Kuang Lin,Shen-Nien Wang,Wen-Hung Wang,Shih-Hsien Hsu,Yu-Hsuan Wu,Ling-Chien Hung,Yen-Hsu Chen,Jin-Ching Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alas1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alas1 antibody (AB115216)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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