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Rabbit Recombinant Monoclonal Alas1 antibody. Mitochondrion marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.


Images

Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860), expandable thumbnail
  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860), expandable thumbnail
  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860), expandable thumbnail
  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products.

Alternative names

ALAS3, ALASH, OK/SW-cl.121, ALAS1, ALAS-H, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, Delta-aminolevulinate synthase 1

Recommended products

Rabbit Recombinant Monoclonal Alas1 antibody. Mitochondrion marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10247
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

'Alas1' also known as 5-Aminolevulinic acid synthase 1 is a mitochondrial enzyme with a mass of approximately 64 kDa. This enzyme plays an important role in heme biosynthesis catalyzing the first step in the pathway by converting glycine and succinyl-CoA to 5-aminolevulinic acid. Unfortunately it does not function alone and often requires the presence of pyridoxal phosphate as a cofactor. 'Alas1' is ubiquitously expressed in a variety of tissues including liver and bone marrow where heme production is critical.

Biological function summary

'Alas1' serves as a rate-limiting enzyme in the heme synthesis pathway. Being the first step it sets the pace for the entire process. It does not form part of any larger protein complex and operates with its required cofactors. Beyond metabolic roles its regulation influences several cellular processes ensuring heme availability precisely matches cellular demand.

Pathways

'Alas1' performs a fundamental role in the mitochondrial heme biosynthesis pathway. This pathway is essential for synthesizing heme a component critical to various cellular functions such as oxygen transport and electron transfer. 'Alas1' interacts with proteins like ferrochelatase the enzyme completing the heme biosynthesis pathway. Coordination between 'Alas1' and other proteins ensures efficient production of heme enabling proper cellular function and adaptation to changes in cellular and systemic conditions.

Associated diseases and disorders

'Alas1' is involved in conditions such as acute intermittent porphyria and certain anemias. Its dysregulation can lead to impaired heme production with downstream effects on cell metabolism and organ function. 'Alas1' has been shown to interact with HMBS (hydroxymethylbilane synthase) a protein linked to different types of porphyria. Understanding its role in these conditions could lead to targeted therapies that modulate its activity to alleviate symptoms or correct underlying biochemical dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    Lanes 1-4: Merged signal (red and green). Green - ab154860 observed at 71 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab154860 Anti-Alas1 antibody [EPR10247] was shown to specifically react with Alas1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ALAS1 knockout HEK-293T cell line ab266473 (knockout cell lysate Human ALAS1 knockout HEK-293T cell lysate ab257348) was used. Wild-type and Alas1 knockout samples were subjected to SDS-PAGE. ab154860 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ALAS1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ALAS1 knockout HEK-293T cell line (Human ALAS1 knockout HEK-293T cell line ab266473)

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: HEK-293 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 71 kDa

    Observed band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution

    All lanes: HepG2 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa, 45 kDa, 71 kDa, 82 kDa

    Observed band size: 31 kDa, 40 kDa, 48 kDa, 71 kDa

  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution

    All lanes: Raji whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa

    Observed band size: 71 kDa

  • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    All lanes: Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: JAR cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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