Rabbit Recombinant Monoclonal ALAS2/ASB antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Images
![Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--immunoprecipitation-img3488.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964)")
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img70454.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964)")
![Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--immunoprecipitation-img159634.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964)")
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img105252.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964)")
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img80890.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (AB184964)")
Publications
Filter by
- Translational psychiatry 13:2692023A role of splenic heme biosynthesis pathway in the persistent prophylactic actions of arketamine in lipopolysaccharide-treated mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Ma,Long Wang,Youge Qu,Xiayun Wan,Kenji HashimotoPubMed 37491335
- Disease markers 2022:12266972022ANTXR1 Regulates Erythroid Cell Proliferation and Differentiation through wnt/-Catenin Signaling Pathway and in Hematopoietic Stem Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Xiangdong Fang,Shengwen Huang,Zhaolin SunPubMed 36065334
- Scientific reports 12:145622022Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTetsuro Ochi,Tohru Fujiwara,Koya Ono,Chie Suzuki,Maika Nikaido,Daichi Inoue,Hiroki Kato,Koichi Onodera,Satoshi Ichikawa,Noriko Fukuhara,Yasushi Onishi,Hisayuki Yokoyama,Yukio Nakamura,Hideo HarigaePubMed 36028755
- Scientific reports 9:196342019Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBibbin T Paul et. al.PubMed 31873120
- Acta pharmaceutica Sinica. B 9:937-9512019Synergistic antitumor activity of artesunate and HDAC inhibitors through elevating heme synthesis synergistic upregulation of ALAS1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCai-Ping Chen et. al.PubMed 31649844
- Molecular genetics and metabolism 128:304-3082019Delta-aminolevulinic acid synthase 2 expression in combination with iron as modifiers of disease severity in erythropoietic protoporphyria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasmin Barman-Aksözen et. al.PubMed 31076252
- Haematologica 104:1756-17672019Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunziata Maio et. al.PubMed 30765471
- Nature communications 9:43862018Long non-coding RNA-dependent mechanism to regulate heme biosynthesis and erythrocyte development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Liu et. al.PubMed 30349036
- Proceedings of the National Academy of Sciences of 114:E8045-E80522017Mutation in human elevates levels of aminolevulinate synthase and protoporphyrin IX to promote erythropoietic protoporphyria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYvette Y Yien et. al.PubMed 28874591
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Target data
Function
Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products (PubMed:14643893, PubMed:21252495, PubMed:21309041, PubMed:21653323, PubMed:32499479). Contributes significantly to heme formation during erythropoiesis (PubMed:2050125). Isoform 3. Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products (PubMed:14643893). Catalytic activity is 75-85% of isoform 1 activity (PubMed:14643893). Isoform 4. Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products (PubMed:14643893). Catalytic activity is 65-75% of isoform 1 activity (PubMed:14643893).
Alternative names
ALASE, ASB, ALAS2, ALAS-E, 5-aminolevulinic acid synthase 2, Delta-ALA synthase 2, Delta-aminolevulinate synthase 2
Recommended products
Rabbit Recombinant Monoclonal ALAS2/ASB antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15112(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALAS2 also known as 5-aminolevulinate synthase 2 is an important enzyme in heme biosynthesis. This enzyme is specific to erythroid cells and it catalyzes the first step in the formation of heme. ALAS2 has an approximate molecular weight of 65 kDa and it is mainly expressed in the mitochondria of erythroblasts in the bone marrow. It functions mechanistically by directing the condensation of glycine and succinyl-CoA to form delta-aminolevulinic acid (ALA) which is important for subsequent steps in heme production.
- Biological function summary
ALAS2 plays a significant role in erythropoiesis as it contributes to heme production which is necessary for hemoglobin assembly. ALAS2 is not known to be part of a complex per se but its function is regulated allosterically by iron availability and activity levels. The synthesis of heme is required for proper formation of red blood cells and ALAS2's regulatory mechanisms ensure that heme is synthesized in synchrony with iron levels.
- Pathways
ALAS2 acts as an initiator of the heme biosynthesis pathway which connects to the larger porphyrin metabolism pathway. This pathway involves additional proteins such as ferrochelatase and uroporphyrinogen III synthase. ALAS2's activity influences the overall efficiency of heme production and in turn iron metabolism. The regulation of ALAS2 by iron levels is a quintessential example of the coordination between cellular metabolism and iron availability in the erythroid lineage.
- Associated diseases and disorders
ALAS2 mutations associate with X-linked sideroblastic anemia a condition characterized by defective heme synthesis in erythroid cells. Relatedly heme biosynthesis dysregulation links to conditions like porphyria. In X-linked sideroblastic anemia malfunction of ALAS2 affects the activity of other enzymes involved in the heme production pathway leading to iron accumulation in mitochondria and ineffective erythropoiesis. Researchers also find connections between ALAS2 and proteins like ferrochelatase in the context of these disorders further impacting the understanding of heme-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--immunoprecipitation-img3488.jpg "Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)")
Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
ab184964 (Purified) at 1:30 dilution (2 µg) immunoprecipitating ALAS2/ASB in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
Lane 2 (+): ab184964 & K-562 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184964 in K-562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184964)All lanes: Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
Predicted band size: 65 kDa
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img70454.jpg "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)")
Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
All lanes: Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 2: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg
Lane 3: Rat brain lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
![Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--immunoprecipitation-img159634.jpg "Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)")
Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab184964 at 1/50 dilution.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
Predicted band size: 65 kDa
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img105252.jpg "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)")
Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
All lanes: Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution
Lane 1: K562 lysate at 20 µg
Lane 2: Jurkat lysate at 20 µg
Lane 3: 293 lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
![Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964), expandable thumbnail](https://content.abcam.com/products/images/alas2-asb-antibody-epr15112b-c-terminal-ab184964--western-blot-img80890.jpg "Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)")
Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
All lanes: Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution
All lanes: Human fetal liver at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 65 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com