Anti-Albumin antibody

• WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin embedded Mouse liver tissue section labelling Albumin with ab106582 at 5 μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling albumin with ab106582 at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with ab106582 at 20 µg/mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

• WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Human liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

• WB

CiteAb

Western blot - Anti-Albumin antibody (AB106582)

Albumin western blotting using Anti-Albumin antibody ab106582. Publication image and figure legend from Tan, Z., Chen, L., et al., 2020, Ann Clin Transl Neurol, PubMed 32302063.

The effect of FK866 on brain edema, and BBB disruption at 1 day after TBI or sham operation. (A) Quantification of brain water content. The bar represents the mean ± SD. n = 6. The bar represents mean ± SD, **p < < 0.01 versus sham, ##p < < 0.01 versus vehicle control. (B) Quantification of Evans blue dye extravasation. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham. ##p < < 0.01 versus vehicle control. (C) Representative western blots and Densitometric quantification of albumin. The bar represents the mean ± SD. N = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group. (D) Representative western blots and densitometric quantification of occluding. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group.

false