Anti-Albumin antibody [EPR12774]
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
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(21 Publications)
Anti-Albumin antibody [EPR12774] (ab192603) is a rabbit monoclonal antibody detecting Albumin in Western Blot, IHC-P. Suitable for Cow, Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
View Alternative Names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [EPR12774] (AB192603)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver labeling Serum albumin with ab192603 at 1/300 dilution. A prediluted HRP Polymer Rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HCT116 (PMID : 24357805, PMID : 28300829, PMID : 19513549), 293T (PMID : 24357805). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti- Bovine Serum Albumin antibody (ab192603) loading control staining at 1/1000 dilution. Exposure time : 48 aeconds
All lanes:
Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (<a href='/en-us/products/primary-antibodies/cystatin-sn-cst1-antibody-epr25933-111-ab307416'>ab307416</a>) at 1/1000 dilution
Lane 1:
MDA-MB-231 (human breast adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg
Lane 2:
SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3:
HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 5:
MDA-MB-231 culture supernatant at 10 µL
Lane 6:
SW480 culture supernatant at 10 µL
Lane 7:
HCT116 culture supernatant at 10 µL
Lane 8:
293T culture supernatant at 10 µL
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 16 kDa
false
Exposure time: 48s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32117064).
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-BSA antibody (ab192603) loading control staining at 1/10000 dilution.
Low expression : placenta (PMID : 32117064).
Samples are non-boiled as boiling may cause protein aggregation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes:
Western blot - Anti-MFSD2A/NLS1 antibody [EPR24503-101] (<a href='/en-us/products/primary-antibodies/mfsd2a-nls1-antibody-epr24503-101-ab307690'>ab307690</a>) at 1/1000 dilution
Lane 1:
Human serum lysate at 20 µg
Lane 2:
Human placenta tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 50 kDa,100 kDa
true
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Protein Z is a glycoprotein of approximately 75 kDa and detected as a 65-kDa band after treated with Peptide : N-glycosidase F (PNGase F). In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-Protein Z antibody [EPR25824-16] (<a href='/en-us/products/primary-antibodies/protein-z-antibody-epr25824-16-ab314743'>ab314743</a>) at 1/1000 dilution
Lane 1:
Untreated human serum lysate at 20 µg
Lane 2:
Human serum tissue lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 75 kDa,65 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : pancreas. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-Protein Z antibody [EPR25824-16] (<a href='/en-us/products/primary-antibodies/protein-z-antibody-epr25824-16-ab314743'>ab314743</a>) at 1/1000 dilution
Lane 1:
Human plasma lysate at 20 µg
Lane 2:
Human serum lysate at 20 µg
Lane 3:
Human pancreas tissue lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 75 kDa
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31324782, 21527525, 22345489). In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-Thrombin antibody [EPR25304-12] (<a href='/en-us/products/primary-antibodies/thrombin-antibody-epr25304-12-ab314480'>ab314480</a>) at 1/1000 dilution
All lanes:
Human plasma lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 50 kDa,60 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Low expression : kidney.
The identity of the bands between 50 kDa and 75 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] - Loading Control (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-C1QA antibody [EPR28764-66] (<a href='/en-us/products/primary-antibodies/c1qa-antibody-epr28764-66-ab315905'>ab315905</a>) at 1/1000 dilution
Lane 1:
Rat plasma lysate at 80 µg
Lane 2:
Rat kidney tissue lysate at 80 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 26 kDa,36 kDa,69 kDa
false
Exposure time: 81s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
All lanes:
Western blot - Anti-Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution
Lane 1:
Mouse kidney at 10 µg
Lane 2:
Mouse spleen at 10 µg
Lane 3:
Rat brain at 10 µg
Lane 4:
Rat kidney at 10 µg
Lane 5:
Rat spleen at 10 µg
Lane 6:
Raw264.7 cell lysate at 10 µg
Lane 7:
PC-12 cell lysate at 10 µg
Lane 8:
NIH-3T3 cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 134 kDa,137 kDa,69 kDa
Observed band size: 69 kDa
false
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-Bovine Serum Albumin antibody ((ab192603) loading control staining at 1/1000 dilution. Exposure time : 180 seconds
All lanes:
Western blot - Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-epr28141-177-serum-loading-control-ab313583'>ab313583</a>) at 1/1000 dilution
All lanes:
Mouse plasma lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 76 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Low expression : liver, kidney.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] - Loading Control (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-C1QA antibody [EPR28764-66] (<a href='/en-us/products/primary-antibodies/c1qa-antibody-epr28764-66-ab315905'>ab315905</a>) at 1/1000 dilution
Lane 1:
Mouse plasma lysate at 20 µg
Lane 2:
Mouse liver tissue lysate at 20 µg
Lane 3:
Mouse serum lysate at 20 µg
Lane 4:
Mouse kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 26 kDa,36 kDa,69 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control ( ab181602) staining at 1/200000 dilution. In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution. Exposure time : 3 seconds
All lanes:
Western blot - Anti-Gentamicin antibody [EPR27991-65] (<a href='/en-us/products/primary-antibodies/gentamicin-antibody-epr27991-65-ab314126'>ab314126</a>) at 1/1000 dilution
Lane 1:
Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2:
RAW264.7 treated with 0.5mg/mL Gentamicin-crosslink-BSA for 4 hours whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
false
Exposure time: 3s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody cross-reacts with recombinant Prostaglandin E1, but not cross-react with recombinant Prostaglandin F1α or Prostaglandin F2α by western blot.
In Western blot, Anti-Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-Prostaglandin E2/1 antibody [EPR25228-27] (<a href='/en-us/products/primary-antibodies/prostaglandin-e2-1-antibody-epr25228-27-ab323269'>ab323269</a>) at 1/1000 dilution
Lane 1:
Prostaglandin E2 cross-linked to BSA at 10 ng
Lane 2:
Prostaglandin E1 cross-linked to BSA at 10 ng
Lane 3:
Prostaglandin F1α cross-linked to BSA at 10 ng
Lane 4:
Prostaglandin F2α cross-linked to BSA at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 67 kDa,69 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
All lanes:
Western blot - Anti-Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution
All lanes:
Bovine Serum albumin at 0.001 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 69 kDa
Observed band size: 69 kDa
false
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
All lanes:
Western blot - Anti-Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution
Lane 1:
HepG2 cell lysate at 20 µg
Lane 2:
Human plasma at 20 µg
Lane 3:
Human serum at 20 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
false
- WB
Supplier Data
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In lane 1, anti-BSA could not detect the total level of cross-linked BSA due to the blocking of epitopes by serotonin.
In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-Serotonin antibody [EPR24431-201] (<a href='/en-us/products/primary-antibodies/serotonin-antibody-epr24431-201-ab315150'>ab315150</a>) at 1/1000 dilution
Lane 1:
Serotonin cross-linked to BSA at 20 ng
Lane 2:
BSA
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 69 kDa
false
Exposure time: 70s
- WB
Lab
Western blot - Anti-Albumin antibody [EPR12774] (AB192603)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody does not cross-react with Oxidized Glutathione(GSSG) by western blot.
In Western blot, Anti-Albumin antibody [EPR12774] - Loading Control (ab192603) (1 : 1000) (67KDa).
All lanes:
Western blot - Anti-Reduced Glutathione(GSH) antibody [EPR26828-37] (<a href='/en-us/products/primary-antibodies/reduced-glutathionegsh-antibody-epr26828-37-ab326336'>ab326336</a>) at 1/1000 dilution
Lane 1:
Reduced Glutathione(GSH) cross-linked to BSA at 10 ng
Lane 2:
Oxidized Glutathione(GSSG) cross-linked to BSA at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 75 kDa,67 kDa
false
Exposure time: 59s
Related conjugates and formulations (4)
-
Anti-Albumin antibody [EPR12774] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Albumin antibody [EPR12774]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Albumin antibody [EPR12774]
-
HRP Anti-Albumin antibody [EPR12774]
Reactivity data
Product details
What is this antibody validated in?
Anti-Albumin antibody [EPR12774] (ab192603) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of Albumin?
Anti-Albumin [EPR12774] (ab192603) specifically detects a band for Albumin (UniProt: P02769) at a molecular weight of 69kDa.
Trusted by the scientific community
Anti-Albumin [EPR12774] (ab192603) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR12774] also available for your convenience: ab192603, Carrier free - ab240330, Alexa Fluor® 488 - ab302577
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Product protocols
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Target data
Publications (21)
Recent publications for all applications. Explore the full list and refine your search
Journal of extracellular biology 4:e70084 PubMed40901048
2025
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Clinical and translational medicine 15:e70198 PubMed39834100
2025
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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 29:215-225 PubMed39806787
2025
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Extracellular vesicles and circulating nucleic acids 5:119-137 PubMed39698410
2024
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Advanced materials (Deerfield Beach, Fla.) 36:e2407156 PubMed39543808
2024
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Scientific reports 13:5425 PubMed37012302
2023
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Disease models & mechanisms 16: PubMed36728410
2023
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Archives of toxicology 96:3349-3361 PubMed36227364
2022
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Chemical research in toxicology 35:1533-1540 PubMed36074022
2022
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Archives of toxicology 96:2967-2981 PubMed35962801
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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