JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175934

Anti-Albumin antibody [EPR12780(B)]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal Albumin antibody. Suitable for WB and reacts with Cow samples. Cited in 4 publications.

View Alternative Names

Albumin, BSA, ALB

1 Images
Western blot - Anti-Albumin antibody [EPR12780(B)] (AB175934)
  • WB

Lab

Western blot - Anti-Albumin antibody [EPR12780(B)] (AB175934)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody [EPR12780(B)] (ab175934) at 1/1000 dilution

All lanes:

Bovine serum albumin lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Albumin antibody [EPR12780(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12780(B)

Isotype

IgG

Carrier free

No

Reacts with

Cow

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB106582

Anti-Albumin antibody

Lab Essentials

Western blot - Anti-Albumin antibody (AB106582)

  • 4
  • 11
Primary Antibodies

AB174658

Biotin Anti-Albumin antibody

  • 0
  • 0
Primary Antibodies

AB112980

Anti-Albumin antibody

Lab Essentials

  • 0
  • 0
Primary Antibodies

AB137885

Anti-Albumin antibody [EPSISR1]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Albumin antibody [EPSISR1] (AB137885)

  • 0
  • 0
Primary Antibodies

AB151742

Anti-Albumin antibody [EPSISR3]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Albumin antibody [EPSISR3] (AB151742)

  • 0
  • 0
Primary Antibodies

AB240109

Anti-Albumin antibody [EPSISR1] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Albumin antibody [EPSISR1] - BSA and Azide free (AB240109)

  • 0
  • 0
Primary Antibodies

AB271979

Anti-Albumin antibody [EPR20195] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - BSA and Azide free (AB271979)

  • 0
  • 0
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
Primary Antibodies

AB222923

Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)

  • 0
  • 0
Primary Antibodies

AB236492

Anti-Albumin antibody [ALB/2144]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [ALB/2144] (AB236492)

  • 0
  • 0
Primary Antibodies

AB112976

HRP Anti-Albumin antibody

  • 0
  • 0

Complementary Products

Assay Kits

AB235628

Albumin (BCG) Assay Kit (Colorimetric)

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52945

Anti-Apolipoprotein A I antibody [EP1368Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)

  • 4
  • 9
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information Albumin
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70052 PubMed40671091

2025

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Liang,Wanlu Zhang,Ao Dungerile,Juping Wang,Runmei Wang

Experimental & molecular medicine 56:583-599 PubMed38424192

2024

Mechanical overload-induced release of extracellular mitochondrial particles from tendon cells leads to inflammation in tendinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Chen,Mengyuan Li,Peilin Chen,Andrew Tai,Jiayue Li,Euphemie Landao Bassonga,Junjie Gao,Delin Liu,David Wood,Brendan F Kennedy,Qiujian Zheng,Ming H Zheng

Journal of extracellular vesicles 11:e12266 PubMed36124834

2022

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Kekoolani S Visan,Richard J Lobb,Sunyoung Ham,Luize G Lima,Carlos Palma,Chai Pei Zhi Edna,Li-Ying Wu,Harsha Gowda,Keshava K Datta,Gunter Hartel,Carlos Salomon,Andreas Möller

Journal of extracellular vesicles 10:e12087 PubMed33936570

2021

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography.

Applications

Unspecified application

Species

Unspecified reactive species

Juan A Martínez-Greene,Karina Hernández-Ortega,Ricardo Quiroz-Baez,Osbaldo Resendis-Antonio,Israel Pichardo-Casas,David A Sinclair,Bogdan Budnik,Alfredo Hidalgo-Miranda,Eileen Uribe-Querol,María Del Pilar Ramos-Godínez,Eduardo Martínez-Martínez
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com