Rabbit Recombinant Monoclonal Albumin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--immunoprecipitation-img10578.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)")
![Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--immunocytochemistry-immunofluorescence-img135742.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)")
![Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--flow-cytometry-intracellular-img25692.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)")
Publications
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- Acta pharmaceutica Sinica. B 12:3281-32972022SYNCRIP controls miR-137 and striatal learning in animal models of methamphetamine abstinence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaeksun Kim,Sung Hyun Tag,Eunjoo Nam,Suji Ham,Sujin Ahn,Juhwan Kim,Doo-Wan Cho,Sangjoon Lee,Young-Su Yang,Seung Eun Lee,Yong Sik Kim,Il-Joo Cho,Kwang Pyo Kim,Su-Cheol Han,Heh-In ImPubMed 35967275
- Cell death discovery 8:772022Long noncoding RNA SNHG1 silencing accelerates hepatocyte-like cell differentiation of bone marrow-derived mesenchymal stem cells to alleviate cirrhosis via the microRNA-15a/SMURF1/UVRAG axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Sun,Xuedong Sun,Sean Hu,Maoqiang Wang,Na Ma,Junhui Chen,Feng DuanPubMed 35194023
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Permeabilization is required. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
2 products for Alternative Version
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
Recommended products
Rabbit Recombinant Monoclonal Albumin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20195
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222923 is the carrier-free version of Anti-Albumin antibody [EPR20195] ab207327.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--immunoprecipitation-img10578.jpg "Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)")
Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with Anti-Albumin antibody [EPR20195] ab207327 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-Albumin antibody [EPR20195] ab207327 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10μg (Input).
Lane 2: Anti-Albumin antibody [EPR20195] ab207327 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Albumin antibody [EPR20195] ab207327 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Albumin antibody [EPR20195] ab207327).
All lanes: Immunoprecipitation - Anti-Albumin antibody [EPR20195] (Anti-Albumin antibody [EPR20195] ab207327)
Predicted band size: 69 kDa
Observed band size: 69 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--immunocytochemistry-immunofluorescence-img135742.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)")
Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with Anti-Albumin antibody [EPR20195] ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: HeLa (PMID: 10476216 and 8314088)).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Albumin antibody [EPR20195] ab207327).
![Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923), expandable thumbnail](https://content.abcam.com/products/images/albumin-antibody-epr20195-low-endotoxin-azide-free-ab222923--flow-cytometry-intracellular-img25692.jpg "Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)")
Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albumin with Anti-Albumin antibody [EPR20195] ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Albumin antibody [EPR20195] ab207327).
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