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Rabbit Recombinant Multiclonal Albumin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (AB320751), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (AB320751), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (AB320751), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [RM1208] (AB320751), expandable thumbnail
  • Western blot - Anti-Albumin antibody [RM1208] (AB320751), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Tested
Tested

Species

Human

Dilution info
1/100
Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

Rabbit Recombinant Multiclonal Albumin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1208

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for mouse ICC

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751)

    Immunohistochemical analysis of paraffin-embedded (A) Rat non-perfused fixed kidney (B) Rat perfused fixed kidney tissue labeling Albumin with ab320751 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma in (A) rat non-perfused fixed kidney, no staining on (B) rat perfused fixed kidney. The section was incubated with ab320751 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751)

    Immunohistochemical analysis of paraffin-embedded (A) Mouse non-perfused fixed kidney (B) Mouse perfused fixed kidney tissue labeling Albumin with ab320751 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma in (A) mouse non-perfused fixed kidney and (B) mouse perfused fixed kidney. The section was incubated with ab320751 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [RM1208] (ab320751)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Albumin with ab320751 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma in human tonsil. The section was incubated with ab320751 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [RM1208] (ab320751)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Albumin with ab320751 at 1/100 (4.87 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: HeLa.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Western blot - Anti-Albumin antibody [RM1208] (ab320751)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Hela (PMID: 10476216 and 8314088) and NIH/3T3 (PMID: 4335066).

    In lane 2 and 4, FBS was removed from the media for few hours before sample preparation for WB as albumin from FBS can affect WB results.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Albumin antibody [RM1208] (ab320751) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa in FBS-free media for 48 hours whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 in FBS-free media for 16 hours whole cell lysate . at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 70 kDa, 36 kDa

    Exposure time: 26s

  • Western blot - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Western blot - Anti-Albumin antibody [RM1208] (ab320751)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Albumin antibody [RM1208] (ab320751) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human skeletal muscle tissue lysate at 20 µg

    Lane 4: Human spleen tissue lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Mouse heart tissue lysate at 20 µg

    Lane 7: Mouse kidney tissue lysate at 20 µg

    Lane 8: Mouse spleen tissue lysate at 20 µg

    Lane 9: Rat brain tissue lysate at 20 µg

    Lane 10: Rat kidney tissue lysate at 20 µg

    Lane 11: Rat heart tissue lysate at 20 µg

    Lane 12: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 68 kDa

    Exposure time: 6s

  • Western blot - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Western blot - Anti-Albumin antibody [RM1208] (ab320751)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1: 6 seconds; Lanes 2-3: 4 seconds

    All lanes: Western blot - Anti-Albumin antibody [RM1208] (ab320751) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Rat liver tissue lysate at 20 µg

    Lane 3: Human plasma lysate at 2 µg

    Lane 4: Rat plasma lysate at 2 µg

    Lane 5: Mouse plasma lysate at 2 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 68 kDa

  • Western blot - Anti-Albumin antibody [RM1208] (ab320751), expandable thumbnail

    Western blot - Anti-Albumin antibody [RM1208] (ab320751)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Hela (PMID: 10476216 and 8314088).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Albumin antibody [RM1208] (ab320751) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 68 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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