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AB279580

Anti-Alcam antibody [001]

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Rabbit Recombinant Monoclonal CD166 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat CD166 antigen.

View Alternative Names

CD166, CD166 antigen, Activated leukocyte cell adhesion molecule, HB2, KG-CAM, Protein MEMD, SB-10 antigen, Alcam

1 Images
Western blot - Anti-Alcam antibody [001] (AB279580)
  • WB

Supplier Data

Western blot - Anti-Alcam antibody [001] (AB279580)

Developed using the Odyssey technique.

Performed under reducing conditions.

All lanes:

Western blot - Anti-Alcam antibody [001] (ab279580) at 1/500 dilution

Lane 1:

SH-SY5Y Whole Cell Lysate at 30 µg

Lane 2:

Daudi Whole Cell Lysate at 30 µg

Lane 3:

HuT78 Whole Cell Lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

001

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat CD166 antigen.

O35112

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALCAM also known as Activated Leukocyte Cell Adhesion Molecule is a protein with a molecular weight of roughly 65-105 kDa due to post-translational modifications. ALCAM expresses in various tissues including the nervous system immune system and endothelial cells. It functions as a cell adhesion molecule and facilitates cell-cell interactions by binding to its ligands like itself and CD6. This interaction plays an important role for maintaining tissue architecture and cell migration.
Biological function summary

The interactions that ALCAM supports are important for processes such as synapse formation and stabilization in the nervous system. ALCAM does not act alone; it participates in complexes with other cell adhesion molecules enhancing signals for tissue development and repair. ALCAM's expression patterns reveal its involvement in leukocyte trafficking and immune responses indicating its importance in maintaining physiological homeostasis.

Pathways

ALCAM plays roles in pathways like the MAPK and PI3K/Akt signaling pathways which are important for cell survival and proliferation. Through these ALCAM interacts with proteins such as ERK1/2 and AKT. These pathways influence cellular responses to external stimuli highlighting the protein's role in growth and differentiation processes. ALCAM's participation in these pathways links it with cellular responses to stress and growth signals.

ALCAM shows associations with cancer metastasis and autoimmune diseases. In cancer changes in ALCAM expression affect tumor progression and metastasis possibly involving interactions with other adhesion molecules and integrins. In autoimmune disorders like multiple sclerosis ALCAM may influence disease progression through interactions with lymphocyte markers. Understanding these connections helps in developing potential therapeutic strategies to modulate ALCAM's function in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
See full target information CD166 antigen
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Product promise

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For full details, please see our Terms & Conditions

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