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AB272281

Anti-Alcam antibody [EPR22341-191] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CD166 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule

1 Images
Sandwich ELISA - Anti-Alcam antibody [EPR22341-191] - BSA and Azide free (Capture) (AB272281)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Alcam antibody [EPR22341-191] - BSA and Azide free (Capture) (AB272281)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270205).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22341-191

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab272281 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ALCAM. The recommended pair for sandwich ELISA is:
Capture: ab272281, Human ALCAM Capture Antibody (unconjugated)
Detector: ab272282, Human ALCAM Detector Antibody (unconjugated)
The reference range value is 0.1875 - 12 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALCAM also known as Activated Leukocyte Cell Adhesion Molecule is a protein with a molecular weight of roughly 65-105 kDa due to post-translational modifications. ALCAM expresses in various tissues including the nervous system immune system and endothelial cells. It functions as a cell adhesion molecule and facilitates cell-cell interactions by binding to its ligands like itself and CD6. This interaction plays an important role for maintaining tissue architecture and cell migration.
Biological function summary

The interactions that ALCAM supports are important for processes such as synapse formation and stabilization in the nervous system. ALCAM does not act alone; it participates in complexes with other cell adhesion molecules enhancing signals for tissue development and repair. ALCAM's expression patterns reveal its involvement in leukocyte trafficking and immune responses indicating its importance in maintaining physiological homeostasis.

Pathways

ALCAM plays roles in pathways like the MAPK and PI3K/Akt signaling pathways which are important for cell survival and proliferation. Through these ALCAM interacts with proteins such as ERK1/2 and AKT. These pathways influence cellular responses to external stimuli highlighting the protein's role in growth and differentiation processes. ALCAM's participation in these pathways links it with cellular responses to stress and growth signals.

ALCAM shows associations with cancer metastasis and autoimmune diseases. In cancer changes in ALCAM expression affect tumor progression and metastasis possibly involving interactions with other adhesion molecules and integrins. In autoimmune disorders like multiple sclerosis ALCAM may influence disease progression through interactions with lymphocyte markers. Understanding these connections helps in developing potential therapeutic strategies to modulate ALCAM's function in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed : 15048703, PubMed : 15496415, PubMed : 16352806, PubMed : 23169771, PubMed : 24945728, PubMed : 7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed : 15048703, PubMed : 16352806, PubMed : 24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed : 15294938, PubMed : 16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed : 15496415, PubMed : 16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed : 29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed : 24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed : 23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed : 15496415, PubMed : 23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).. Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
See full target information ALCAM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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