AB231521

Anti-Alcohol Dehydrogenase antibody

Name: Anti-Alcohol Dehydrogenase antibody (ab231521) | Abcam
Brand: Abcam Limited
SKU: AB231521
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Alcohol Dehydrogenase antibody. Suitable for WB, IHC-P and reacts with Rat, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADH1A aa 150 to C-terminus.

View Alternative Names

ADH1, ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Paraffin-embedded human stomach cancer tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Paraffin-embedded human lung cancer tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

araffin-embedded human stomach tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Paraffin-embedded human breast cancer tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Paraffin-embedded human skin cancer tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Paraffin-embedded human liver tissue stained for Alcohol Dehydrogenase using ab231521 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

All lanes:

Western blot - Anti-Alcohol Dehydrogenase antibody (ab231521) at 2 µg/mL

All lanes:

Recombinant human Alcohol Dehydrogenase protein

Predicted band size: 23 kDa,40 kDa

false

Supplier Data

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

All lanes:

Western blot - Anti-Alcohol Dehydrogenase antibody (ab231521) at 1.5 µg/mL

All lanes:

Rat liver lysate

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

All lanes:

Western blot - Anti-Alcohol Dehydrogenase antibody (ab231521) at 2 µg/mL

All lanes:

Pig liver lysate

Predicted band size: 120 kDa,40 kDa,47 kDa,51 kDa,55 kDa,65 kDa,67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ADH1A aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.

Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alcohol dehydrogenase (PubMed : 2738060). Oxidizes primary as well as secondary alcohols. Ethanol is a very poor substrate (PubMed : 2738060).

See full target information ADH1A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of vascular research 55:255-267 PubMed30179877

2018

Perfusion Tissue Culture Initiates Differential Remodeling of Internal Thoracic Arteries, Radial Arteries, and Saphenous Veins.

Applications

Unspecified application

Species

Unspecified reactive species

David A Prim,Vinal Menon,Shahd Hasanian,Laurel Carter,Tarek Shazly,Jay D Potts,John F Eberth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alcohol Dehydrogenase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody (AB231521)

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

Western blot - Anti-Alcohol Dehydrogenase antibody (AB231521)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise