AB108203

Anti-Alcohol Dehydrogenase antibody [EPR4439]

Name: Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) | Abcam
Brand: Abcam Limited
SKU: AB108203
Rating: 5 (6 reviews)

RabMAb
Recombinant
What is this?

(6 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal Alcohol Dehydrogenase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

ADH1, ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.

Unknown

Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

All lanes:

Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Mouse liver tissue lysate at 10 µg

Lane 3:

Human fetal liver tissue lysate at 10 µg

Lane 4:

Rat liver tissue lysate at 10 µg

Predicted band size: 40 kDa

false

ICC/IF

AbReview45992****

Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).

This image is courtesy of an Abreview submitted by Armen Petrosyan

HRP

HRP Anti-Alcohol Dehydrogenase antibody [EPR4439]
Carrier free

Anti-Alcohol Dehydrogenase antibody [EPR4439] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4439

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.

Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Alcohol dehydrogenase (PubMed : 2738060). Oxidizes primary as well as secondary alcohols. Ethanol is a very poor substrate (PubMed : 2738060).

See full target information ADH1A

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:32986 PubMed41006587

2025

Overexpression of alcohol dehydrogenase 1 A inhibits the progress of triple negative breast cancer via Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Su,Chunlin Qiao,Jin Luo,Bianling Zhu,Yunxiao Liu

Experimental and therapeutic medicine 25:169 PubMed37006880

2023

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gao,Shiqi Suo,Jingxia Li,Chunxia Wang,Ranran Deng,Yuxia Hu,Chunzheng Zhang

Cell 179:561-577.e22 PubMed31585088

2019

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Gao,Hongwen Zhu,Liangqing Dong,Weiwei Shi,Ran Chen,Zhijian Song,Chen Huang,Junqiang Li,Xiaowei Dong,Yanting Zhou,Qian Liu,Lijie Ma,Xiaoying Wang,Jian Zhou,Yansheng Liu,Emily Boja,Ana I Robles,Weiping Ma,Pei Wang,Yize Li,Li Ding,Bo Wen,Bing Zhang,Henry Rodriguez,Daming Gao,Hu Zhou,Jia Fan

The Journal of clinical investigation 129:5424-5441 PubMed31487269

2019

Schwann cells expressing nociceptive channel TRPA1 orchestrate ethanol-evoked neuropathic pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco De Logu,Simone Li Puma,Lorenzo Landini,Francesca Portelli,Alessandro Innocenti,Daniel Souza Monteiro de Araujo,Malvin N Janal,Riccardo Patacchini,Nigel W Bunnett,Pierangelo Geppetti,Romina Nassini

Journal of cellular biochemistry 120:15007-15017 PubMed31056772

2019

The retinol-retinoic acid metabolic pathway is impaired in the lumbar spine of a rat model of congenital kyphoscoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Sonoda,Haku Iizuka,Sho Ishiwata,Daisuke Tsunoda,Masako Abe,Kenji Takagishi,Hirotaka Chikuda,Noriyuki Koibuchi,Noriaki Shimokawa

Molecular cancer research : MCR 17:225-237 PubMed30224543

2018

The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Manca,Cole P Frisbie,Chad A LaGrange,Carol A Casey,Jean-Jack M Riethoven,Armen Petrosyan

ACS pharmacology & translational science 1:50-60 PubMed31696159

2018

Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Monideepa Sengupta,Kristine Griffett,Colin A Flaveny,Thomas P Burris

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alcohol Dehydrogenase antibody [EPR4439]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (AB108203)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise