JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB184171

Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)]

3

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal Aldehyde dehydrogenase 10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ALDH10, FALDH, ALDH3A2, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)

Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain : DAPI (blue).

Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixedHepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control : Rabbit monoclonal IgG (green).

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)
  • WB

Supplier Data

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)

All lanes:

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)
  • WB

Lab

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)

Lanes 1 - 2 : Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type HeLa and ALDH3A2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ALDH3A2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-aldh3a2-aldehyde-dehydrogenase-10-knockout-hela-cell-line-ab265427'>ab265427</a>)

Predicted band size: 55 kDa

Observed band size: 50 kDa

false

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)
  • WB

Supplier Data

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171)

All lanes:

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution

All lanes:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

  • Carrier free

    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15425(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB155893

ALDH Activity Assay Kit (Colorimetric)

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75060

Anti-GNPAT/DAPAT antibody

Western blot - Anti-GNPAT/DAPAT antibody (AB75060)

  • 4
  • 1
Primary Antibodies

AB190685

Anti-GCLC antibody [EP13475]

RabMAb|Recombinant

Immunoprecipitation - Anti-GCLC antibody [EP13475] (AB190685)

  • 5
  • 7
Primary Antibodies

AB76148

Anti-MSI2 antibody [EP1305Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [EP1305Y] (AB76148)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/170", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldehyde dehydrogenase 10 (ALDH10) functions mechanically as an enzyme that catalyzes the NAD(P)+-dependent oxidation of aldehydes to carboxylic acids. It belongs to a larger family of aldehyde dehydrogenases. Frequently used alternate names include ALDH3A2 ensuring clarification across research contexts. The mass of ALDH10 can vary slightly depending on specific isoforms and protein modifications but typically his family is around 50-55 kDa. Expression of ALDH10 mainly occurs in the liver and also in tissues including kidney and lung reflecting its roles in aldehyde metabolism across different tissues.
Biological function summary

Aldehyde dehydrogenase 10 participates in the detoxification of aldehydes which are generated during metabolic processes. It plays an important role in protecting cells from oxidative stress by converting reactive aldehydes to less toxic carboxylic acids. ALDH10 does not seem to form part of a larger complex but acts independently to perform its function. Its enzymatic activity impacts cellular redox balance important for maintaining cellular health and function.

Pathways

Aldehyde dehydrogenase 10 plays a significant role in the metabolic pathway processes involving amino acid and fatty acid metabolism. It is important in metabolizing aldehydes generated through lipid peroxidation and amino acid degradation pathways contributing to maintaining metabolic homeostasis. ALDH10 connects functionally with other aldehyde dehydrogenase family members like ALDH2 and ALDH3A2 which collaborate in handling various aldehyde substrates critical to these pathways.

Aldehyde dehydrogenase 10 holds relevance to conditions like Sjögren-Larsson syndrome and non-alcoholic fatty liver disease. ALDH3A2 a closely related protein links directly with Sjögren-Larsson syndrome due to its known genetic mutations causing reduced enzymatic function. The role of ALDH10 in modulating liver metabolism links it to non-alcoholic fatty liver disease as altered detoxification capability can lead to disease progression. Understanding these relationships helps in targeting therapies that might mitigate disease impacts by enhancing detoxification pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed : 18035827, PubMed : 18182499, PubMed : 22633490, PubMed : 25047030, PubMed : 9133646, PubMed : 9662422). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed : 22633490).
See full target information ALDH3A2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2712 PubMed38548729

2024

Bioorthogonal photocatalytic proximity labeling in primary living samples.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Liu,Fuhu Guo,Yufan Zhu,Shengnan Qin,Yuchen Hou,Haotian Guo,Feng Lin,Peng R Chen,Xinyuan Fan

The Journal of cell biology 221: PubMed36053215

2022

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Fengping Feng,Han Feng,Pengkai Hu,Yanhong Xue,Tao Xu,Eli Song
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com