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Rabbit Recombinant Monoclonal Aldehyde dehydrogenase 10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.


Images

Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171), expandable thumbnail
  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171), expandable thumbnail
  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (AB184171), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/170
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:18035827, PubMed:18182499, PubMed:22633490, PubMed:25047030, PubMed:9133646, PubMed:9662422). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:22633490).

Alternative names

ALDH10, FALDH, ALDH3A2, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase

Recommended products

Rabbit Recombinant Monoclonal Aldehyde dehydrogenase 10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15425(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aldehyde dehydrogenase 10 (ALDH10) functions mechanically as an enzyme that catalyzes the NAD(P)+-dependent oxidation of aldehydes to carboxylic acids. It belongs to a larger family of aldehyde dehydrogenases. Frequently used alternate names include ALDH3A2 ensuring clarification across research contexts. The mass of ALDH10 can vary slightly depending on specific isoforms and protein modifications but typically his family is around 50-55 kDa. Expression of ALDH10 mainly occurs in the liver and also in tissues including kidney and lung reflecting its roles in aldehyde metabolism across different tissues.

Biological function summary

Aldehyde dehydrogenase 10 participates in the detoxification of aldehydes which are generated during metabolic processes. It plays an important role in protecting cells from oxidative stress by converting reactive aldehydes to less toxic carboxylic acids. ALDH10 does not seem to form part of a larger complex but acts independently to perform its function. Its enzymatic activity impacts cellular redox balance important for maintaining cellular health and function.

Pathways

Aldehyde dehydrogenase 10 plays a significant role in the metabolic pathway processes involving amino acid and fatty acid metabolism. It is important in metabolizing aldehydes generated through lipid peroxidation and amino acid degradation pathways contributing to maintaining metabolic homeostasis. ALDH10 connects functionally with other aldehyde dehydrogenase family members like ALDH2 and ALDH3A2 which collaborate in handling various aldehyde substrates critical to these pathways.

Associated diseases and disorders

Aldehyde dehydrogenase 10 holds relevance to conditions like Sjögren-Larsson syndrome and non-alcoholic fatty liver disease. ALDH3A2 a closely related protein links directly with Sjögren-Larsson syndrome due to its known genetic mutations causing reduced enzymatic function. The role of ALDH10 in modulating liver metabolism links it to non-alcoholic fatty liver disease as altered detoxification capability can lead to disease progression. Understanding these relationships helps in targeting therapies that might mitigate disease impacts by enhancing detoxification pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171), expandable thumbnail

    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    Lanes 1 - 2:Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
    ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line ab265427 (ALDH3A2 knockout cell lysate Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell lysate ab257829). Wild-type HeLa and ALDH3A2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ALDH3A2 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line (Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line ab265427)

    Performed under reducing conditions.

    Predicted band size: 55 kDa

    Observed band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixedHepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171), expandable thumbnail

    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    All lanes: Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution

    All lanes: U87-MG cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171), expandable thumbnail

    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    All lanes: Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution

    Lane 1: A549 cell lysate at 20 µg

    Lane 2: 293T cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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