AB197828

Anti-Aldehyde Oxidase antibody - C-terminal

Name: Anti-Aldehyde Oxidase antibody - C-terminal (ab197828) | Abcam
Brand: Abcam Limited
SKU: AB197828
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Aldehyde Oxidase antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Fusion protein corresponding to AOX1, fused to AOX1.

View Alternative Names

AO, AOX1, Aldehyde oxidase, Aldehyde oxidase 1, Azaheterocycle hydroxylase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde Oxidase antibody - C-terminal (AB197828)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde Oxidase antibody - C-terminal (AB197828)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling Aldehyde Oxidase with ab197828 at 1/65.

Supplier Data

Western blot - Anti-Aldehyde Oxidase antibody - C-terminal (AB197828)

Gel : 10% SDS-PAGE

All lanes:

Western blot - Anti-Aldehyde Oxidase antibody - C-terminal (ab197828) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 40 µg

Predicted band size: 148 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Fusion protein corresponding to AOX1, fused to AOX1. The exact immunogen used to generate this antibody is proprietary information.

Q06278

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/300", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldehyde Oxidase (AOX) also known by alternate names such as aldehyde oxidoreductase is a cytosolic enzyme with a molecular mass of approximately 150 kDa. This enzyme expresses itself across various tissues including the liver lungs and other organs where it catalyzes the oxidation of aldehydes into acids. AOX requires molybdenum iron and flavin adenine dinucleotide (FAD) as cofactors enabling its activity in the detoxification of xenobiotic and endogenous aldehydes.

Biological function summary

Aldehyde oxidation involves processes that transform aldehydes into less reactive carboxylic acids aiding cellular regulatory functions. AOX plays a role within the molybdo-flavoenzyme complex. This complex participates in metabolic reactions that help maintain homeostasis within cells and manage oxidative stress. Its presence in the liver highlights its role in drug metabolism where it manages the oxidation of a variety of substances and reduces metabolic burden.

Pathways

One of the significant roles of aldehyde oxidase lies within the purine metabolism pathway. It helps convert purines into uric acid which supports the final steps of purine degradation. This enzyme shares pathways with xanthine oxidase another critical enzyme in purine catabolism. Additionally AOX participates in the biosynthesis and breakdown of several drugs and xenobiotics in the drug metabolism pathway exhibiting similarity to cytochrome P450 enzymes in its substrate preferences.

Aldehyde oxidase's dysfunction links to disorders like liver damage and certain neurological conditions due to its role in oxidative stress management. Impaired activity of AOX can lead to toxic aldehyde accumulation creating a harmful cellular environment that exacerbates liver damage. Furthermore AOX's connection with other enzymes such as xanthine oxidase relates it to conditions like gout where altered purine metabolism leads to excessive uric acid production and crystal formation in joints.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. May also catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.

See full target information AOX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Drug metabolism and disposition: the biological fa 45:883-886 PubMed28487309

2017

Molecular Cloning and Characterization of Marmoset Aldehyde Oxidase.

Applications

Unspecified application

Species

Unspecified reactive species

Shotaro Uehara,Yasuhiro Uno,Eriko Okamoto,Takashi Inoue,Erika Sasaki,Hiroshi Yamazaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldehyde Oxidase antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde Oxidase antibody - C-terminal (AB197828)

Western blot - Anti-Aldehyde Oxidase antibody - C-terminal (AB197828)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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