Rabbit Polyclonal ALDH1A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1.
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Publications
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- Molecular biology reports 50:5273-52822023The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSzandra Schwarcz,Patrik Kovács,Tünde Kovács,Gyula Ujlaki,Petra Nyerges,Karen Uray,Péter Bai,Edit MikóPubMed 37145211
- Cell death & disease 13:1572022Long non-coding ROR promotes the progression of papillary thyroid carcinoma through regulation of the TESC/ALDH1A1/TUBB3/PTEN axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxia Fan,Xin Fan,Hao Yan,Zheng Liu,Xiaoming Wang,Qingling Yuan,Jie Xie,Xiubo Lu,Yang YangPubMed 35173149
- Clinical proteomics 18:272021Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMamatha Bhat,Sergi Clotet-Freixas,Cristina Baciu,Elisa Pasini,Ahmed Hammad,Tommy Ivanics,Shelby Reid,Amirhossein Azhie,Marc Angeli,Anand Ghanekar,Sandra Fischer,Gonzalo Sapisochin,Ana KonvalinkaPubMed 34794390
- Investigative ophthalmology & visual science 61:192020S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulin Wang,Guiping Gao,Ying Wu,Yuqin Wang,Xiaorong Wu,Qiong ZhouPubMed 32926102
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALDH1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P00352
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Antigen retrieval: EDTA-based, pH 8.0 buffer, 15 minutes. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:19296407, PubMed:25450233, PubMed:26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).
Alternative names
ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1
Recommended products
Rabbit Polyclonal ALDH1A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALDH1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P00352
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.
- Biological function summary
ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.
- Pathways
The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.
- Associated diseases and disorders
ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab227948)
Paraffin-embedded Cal27 xenograft tissue stained for ALDH1A1 using ab227948 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based, pH 8.0 buffer, 15 minutes.
Western blot - Anti-ALDH1A1 antibody (ab227948)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution
All lanes: Rat liver lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (ab227948)
Methanol-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227948 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
Western blot - Anti-ALDH1A1 antibody (ab227948)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/10000 dilution
All lanes: Mouse liver lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
Western blot - Anti-ALDH1A1 antibody (ab227948)
10% SDS PAGE gel.
All lanes: Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution
All lanes: A549 (human lung carcinoma cell line) whole cell lysate) at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
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