AB227948

Anti-ALDH1A1 antibody

Name: Anti-ALDH1A1 antibody (ab227948) | Abcam
Brand: Abcam Limited
SKU: AB227948

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(7 Publications)

Rabbit Polyclonal ALDH1A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Cat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1.

View Alternative Names

ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227948)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227948)

Methanol-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227948 at 1/500 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227948)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227948)

Paraffin-embedded Cal27 xenograft tissue stained for ALDH1A1 using ab227948 at 1/500 dilution in immunohistochemical analysis.

Antigen retrieval : EDTA-based, pH 8.0 buffer, 15 minutes.

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227948)

10% SDS PAGE gel.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate) at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227948)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution

All lanes:

Rat liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227948)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/10000 dilution

All lanes:

Mouse liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227948)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution

All lanes:

PG-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cat, Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALDH1A1. The exact immunogen used to generate this antibody is proprietary information.

P00352

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval: EDTA-based, pH 8.0 buffer, 15 minutes.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed : 12941160, PubMed : 15623782, PubMed : 17175089, PubMed : 19296407, PubMed : 25450233, PubMed : 26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed : 12941160, PubMed : 15623782, PubMed : 19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed : 17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

See full target information ALDH1A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1487685 PubMed39723238

2024

Anticarcinogenic effects of ursodeoxycholic acid in pancreatic adenocarcinoma cell models.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Kovács,Szandra Schwarcz,Petra Nyerges,Tímea Ingrid Bíró,Gyula Ujlaki,Péter Bai,Edit Mikó

Cell death discovery 10:248 PubMed38782891

2024

The bacterial metabolite, lithocholic acid, has antineoplastic effects in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Szandra Schwarcz,Patrik Kovács,Petra Nyerges,Gyula Ujlaki,Adrienn Sipos,Karen Uray,Péter Bai,Edit Mikó

Journal of cellular and molecular medicine 28:e18395 PubMed38774995

2024

Analysis of the effects of M2 macrophage-derived PDE4C on the prognosis, metastasis and immunotherapy benefit of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Runsang Pan,Rui Hu,Hao Zhang,Shan Lei,Lu Zhang,Changhua Zhou,Zhirui Zeng,Xiaobin Tian,Quan Xie

Molecular biology reports 50:5273-5282 PubMed37145211

2023

The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models.

Applications

Unspecified application

Species

Unspecified reactive species

Szandra Schwarcz,Patrik Kovács,Tünde Kovács,Gyula Ujlaki,Petra Nyerges,Karen Uray,Péter Bai,Edit Mikó

Cell death & disease 13:157 PubMed35173149

2022

Long non-coding ROR promotes the progression of papillary thyroid carcinoma through regulation of the TESC/ALDH1A1/TUBB3/PTEN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxia Fan,Xin Fan,Hao Yan,Zheng Liu,Xiaoming Wang,Qingling Yuan,Jie Xie,Xiubo Lu,Yang Yang

Clinical proteomics 18:27 PubMed34794390

2021

Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan.

Applications

Unspecified application

Species

Unspecified reactive species

Mamatha Bhat,Sergi Clotet-Freixas,Cristina Baciu,Elisa Pasini,Ahmed Hammad,Tommy Ivanics,Shelby Reid,Amirhossein Azhie,Marc Angeli,Anand Ghanekar,Sandra Fischer,Gonzalo Sapisochin,Ana Konvalinka

Investigative ophthalmology & visual science 61:19 PubMed32926102

2020

S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Wang,Guiping Gao,Ying Wu,Yuqin Wang,Xiaorong Wu,Qiong Zhou

View all publications

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Product promise

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Anti-ALDH1A1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227948)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227948)

Western blot - Anti-ALDH1A1 antibody (AB227948)

Western blot - Anti-ALDH1A1 antibody (AB227948)

Western blot - Anti-ALDH1A1 antibody (AB227948)

Western blot - Anti-ALDH1A1 antibody (AB227948)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise