Skip to main content

Mouse Monoclonal ALDH1A1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1 aa 300-450.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (AB219303), expandable thumbnail
  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (AB219303), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (AB219303), expandable thumbnail
  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (AB219303), expandable thumbnail
  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (AB219303), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ALDH1A1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link P00352

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

(Primary incubation for 30 minutes at RT).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:19296407, PubMed:25450233, PubMed:26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

Alternative names

Recommended products

Mouse Monoclonal ALDH1A1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1 aa 300-450.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human ALDH1A1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link P00352
Clone number

ALDH1A1/1381

Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

ab219303 was purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

Associated diseases and disorders

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling ALDH1A1 with ab219303 at 1 μg/ml.

  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303), expandable thumbnail

    Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303)

    All lanes: Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303) at 1 µg/mL

    Lane 1: K562 cell lysate

    Lane 2: Human lung lysate

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testicular carcinoma tissue labeling ALDH1A1 with ab219303 at 1 μg/ml.

  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303), expandable thumbnail

    Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303)

    Western blot analysis of human liver tissue lysate labeling ALDH1A1 with ab219303 at 1 ug/ml.

    All lanes: Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303) at 1 µg/mL

    All lanes: Human liver tissue lysate

    Predicted band size: 54 kDa

  • Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303), expandable thumbnail

    Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303)

    Western blot: Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab219303 was shown to bind specifically to ALDH1A1. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in ALDH1A1 knockout cell line. To generate this image, wild-type and ALDH1A1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (ab219303) at 1 µg/mL

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ALDH1A1 knockout A549 cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com