Name: Anti-ALDH1A1 antibody [EP1933Y] - C-terminal
SKU: ab52492
Rating: 4 (22 reviews)

Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) is a rabbit monoclonal antibody that is used to detect ALDH1A1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse samples.

- Specificity confirmed with ALDH1A1 knockout cell line validation

Related conjugates and formulations

ab209437
Conjugated
PE Anti-ALDH1A1 antibody [EP1933Y]
ab195517
Conjugated
HRP Anti-ALDH1A1 antibody [EP1933Y]
ab206884
Conjugated
Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y]
ab195254
Conjugated
Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y]
ab195255
Conjugated
Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y]
ab215996
Carrier free
Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free

Images

Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (AB52492), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20 - 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500 - 1/2000	Notes -
Species Human	Dilution info 1/500 - 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes For unpurified use at 1/1000. Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information ALDH1A1

Function

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:19296407, PubMed:25450233, PubMed:26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

Alternative names

ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1933Y
Purification technique: Affinity purification Protein A
Specificity: The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of ALDH1A1?

Anti-ALDH1A1 [EP1933Y] - C-terminal (ab52492) specifically detects a band for ALDH1A1 (UniProt: P00352) at a molecular weight of 55kDa.

Trusted by the scientific community

Anti-ALDH1A1 [EP1933Y] - C-terminal (ab52492) was first used in a scientific publication in 2007 and has been cited over 180 times in peer-reviewed journals.

Reviewed by scientists

Anti-ALDH1A1 [EP1933Y] - C-terminal (ab52492) has over 20 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) has been confirmed by Western blot testing in ALDH1A1 Knockout A549 cells.

Other related products

We have a range of other formats of antibody clone [EP1933Y] also available for your convenience:
ab52492, Alexa Fluor® 488 - ab195254, Alexa Fluor® 647 - ab195255, HRP - ab195517, Alexa Fluor® 594 - ab206884, PE - ab209437, Carrier free - ab215996

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

Associated diseases and disorders

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
ab52492 (purified) at 1/20 dilution (2μg) immunoprecipitating ALDH1A1 in HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates.
Lane 1: HepG2 whole cell lysates 10μg.
Lane 2: ab52492 IP in HepG2 whole cell lysates.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52492 in HepG2 whole cell lysates.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Predicted band size: 54 kDa
Image from Gong et al PLoS One. 2010 Dec 20;5(12):e15630. doi: 10.1371/journal.pone.0015630. Fig 1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Tumor tissues of primary invasive ductal carcinomas of the breast were obtained from 192 female patients with stage IIB and III prior to pre-operative neoadjuvant chemotherapy.
(progressive or stable disease, PD/SD)
(partial or complete remission, PR/CR)
The level of ALDH1 was tested by immunohistochemistry staining in paraffin-embedded tissue sections. Rabbit monoclonal ALDH1A1 antibody (unpurified ab52492, Abcam) used at a 1:100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ALDH1A1 with purified ab52492 at 1/50 dilution (3.54 μg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
All lanes: Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: Human small intestine lysates
Lane 3: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 4: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 5: Mouse liver lysates at 20 µg
Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ALDH1A1 with ab52492 (purified) at 1/500 dilution (4 μg/ml).
Cells were fixed in 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 dilution (2 μg/ml). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)was used to counterstain at 1/200 dilution (2.5 μg/ml). DAPI was used as nuclear counterstain.
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: No staining on MCF-7 cell line.
Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: Human small intestine tissue lysate at 15 µg
Lane 3: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 4: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 5: Mouse liver tissue lysate at 15 µg
Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 1min
Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Overlay histogram showingHepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab52492 (unpurified) (red line).
The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluorr^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunohistochemical analysis of paraffin-embedded human liver tissue sections labeling ALDH1A1 with ab52492 (unpurified) at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human brain tissue sections labeling ALDH1A1 with ab52492 (unpurified).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human lung tissue sections labeling ALDH1A1 with ab52492 (unpurified).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human kidney tissue sections labeling ALDH1A1 with ab52492 (unpurified).
Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Western blot: Anti-ALDH1A1 antibody [EP1933Y] (ab52492) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab52492 was shown to bind specifically to ALDH1A1. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in ALDH1A1 knockout cell line. To generate this image, wild-type and ALDH1A1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ALDH1A1 knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human ALDH1A1 knockout A549 cell line (Human ALDH1A1 knockout A549 cell line ab261864)
Lane 2: Western blot - Human ALDH1A1 knockout A549 cell lysate (Human ALDH1A1 knockout A549 cell lysate ab261673)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ALDH1A1 with purified ab52492 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).