AB75674

Anti-ALDH1A2 antibody

Name: Anti-ALDH1A2 antibody (ab75674) | Abcam
Brand: Abcam Limited
SKU: AB75674
Rating: 4 (8 reviews)

BOND RX™ Validated
What is this?

(8 Reviews)

(14 Publications)

Rabbit Polyclonal ALDH1A2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

RALDH2, ALDH1A2, Retinal dehydrogenase 2, RALDH 2, RalDH2, Aldehyde dehydrogenase family 1 member A2, Retinaldehyde-specific dehydrogenase type 2, RALDH(II)

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB75674)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB75674)

IHC image of ab75674 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-ALDH1A2 antibody (AB75674)

All lanes:

Western blot - Anti-ALDH1A2 antibody (ab75674) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 17 kDa,46 kDa,56 kDa,73 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A2 also known as Retinaldehyde Dehydrogenase 2 (RALDH2) is an enzyme that plays an important role in the metabolic conversion of retinaldehyde to retinoic acid a bioactive form of vitamin A. It has a known mass of approximately 56 kDa. ALDH1A2 has high expression in multiple tissues including the liver pancreas and various types of epithelial cells. This enzyme is cytosolic actively contributing to local retinoid metabolism.

Biological function summary

ALDH1A2 is essential in embryonic development and tissue differentiation. It acts by regulating the levels of retinoic acid necessary for directing gene expression. ALDH1A2 activity is not known to be part of a complex but interacts with other retinoid-binding proteins to achieve its functions. It orchestrates cellular processes by modulating growth factors that are sensitive to retinoic acid.

Pathways

ALDH1A2 is a participant in the retinol metabolism pathway and the broader RA-signaling pathway. It ensures the generation of retinoic acid critical for gene regulation within these pathways. RALDH2's activity is closely related to other dehydrogenases and aldehyde oxidases that contribute to retinoid homeostasis. The interplay with these proteins emphasizes its role in ensuring a balanced retinoic acid supply.

ALDH1A2 has been implicated in conditions such as congenital diaphragmatic hernia and certain cancer types like breast cancer. Aberrant regulation or mutations in ALDH1A2 can lead to altered retinoic acid levels affecting cell proliferation and differentiation pathways. Networks involving ALDH1A2 with proteins like CYP26B1 responsible for retinoic acid catabolism highlight its involvement in these medical conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively (PubMed : 29240402, PubMed : 33565183). Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable) (PubMed : 33565183). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein (By similarity). Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal (By similarity). Displays complete lack of activity with citral (By similarity).

See full target information ALDH1A2

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Science translational medicine 14:eabm4054 PubMed36542696

2022

Variants in reveal an anti-inflammatory role for retinoic acid and a new class of disease-modifying drugs in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Linyi Zhu,Pragash Kamalathevan,Lada A Koneva,Jadwiga Miotla Zarebska,Anastasios Chanalaris,Heba Ismail,Akira Wiberg,Michael Ng,Hayat Muhammad,John Walsby-Tickle,James S O McCullagh,Fiona E Watt,Stephen N Sansom,Dominic Furniss,Matthew D Gardiner,Tonia L Vincent,Nick Riley,Michelle Spiteri,Ian McNab,Christopher Little,Lucy Cogswell,Paul Critchley,Henk Giele,Rebecca Shirley

Development (Cambridge, England) 149: PubMed35020897

2022

FGFR2 signaling enhances the SHH-BMP4 signaling axis in early ureter development.

Applications

Unspecified application

Species

Unspecified reactive species

Max Meuser,Lena Deuper,Carsten Rudat,Nurullah Aydoğdu,Hauke Thiesler,Patricia Zarnovican,Herbert Hildebrandt,Mark-Oliver Trowe,Andreas Kispert

Science advances 6: PubMed33148658

2020

Histone demethylase LSD1 is critical for endochondral ossification during bone fracture healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Heng Feng,Wenhui Xing,Yujiao Han,Jinlong Suo,Alisha R Yallowitz,Niandong Qian,Yujiang Shi,Matthew B Greenblatt,Weiguo Zou

Frontiers in cell and developmental biology 8:94 PubMed32258025

2020

Inhibition of GSK3 Represses the Expression of Retinoic Acid Synthetic Enzyme ALDH1A2 via Wnt/β-Catenin Signaling in WiT49 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Li,Hui Gong,Jiangfeng Ding,Fujuan Zhao,Jihui Du,Jun Wan,Juan Zhang,Shaoxiong Liu,Jing Li,Lei Wang,Bei Zhou

eNeuro 7: PubMed31879367

2019

Retinoic Acid Is Required for Oligodendrocyte Precursor Cell Production and Differentiation in the Postnatal Mouse Corpus Callosum.

Applications

Unspecified application

Species

Unspecified reactive species

Vivianne E Morrison,Victoria N Smith,Jeffrey K Huang

mSphere 4: PubMed31167948

2019

Vitamin A Metabolism by Dendritic Cells Triggers an Antimicrobial Response against Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elliot W Kim,Avelino De Leon,Zhichun Jiang,Roxana A Radu,Adrian R Martineau,Edward D Chan,Xiyuan Bai,Wen-Lin Su,Dennis J Montoya,Robert L Modlin,Philip T Liu

Developmental dynamics : an official publication o 247:924-933 PubMed29708625

2018

Role of the Wilms' tumor suppressor gene Wt1 in pancreatic development.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Ariza,Ana Cañete,Anabel Rojas,Ramón Muñoz-Chápuli,Rita Carmona

Disease models & mechanisms 11: PubMed29361518

2018

The IgCAM CLMP regulates expression of Connexin43 and Connexin45 in intestinal and ureteral smooth muscle contraction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Langhorst,René Jüttner,Dieter Groneberg,Azadeh Mohtashamdolatshahi,Laura Pelz,Bettina Purfürst,Kai M Schmidt-Ott,Andreas Friebe,Fritz G Rathjen

Brain research 1679:155-170 PubMed29217155

2017

Pleiotropic neuropathological and biochemical alterations associated with Myo5a mutation in a rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin K Landrock,Patti Sullivan,Heidi Martini-Stoica,David S Goldstein,Brett H Graham,Shinya Yamamoto,Hugo J Bellen,Richard A Gibbs,Rui Chen,Marcello D'Amelio,George Stoica

PLoS genetics 13:e1007068 PubMed29084269

2017

Non-muscle myosin IIB (Myh10) is required for epicardial function and coronary vessel formation during mammalian development.

Applications

Unspecified application

Species

Unspecified reactive species

Liam A Ridge,Karen Mitchell,Ali Al-Anbaki,Wasay Mohiuddin Shaikh Qureshi,Louise A Stephen,Gennadiy Tenin,Yinhui Lu,Irina-Elena Lupu,Christopher Clowes,Abigail Robertson,Emma Barnes,Jayne A Wright,Bernard Keavney,Elisabeth Ehler,Simon C Lovell,Karl E Kadler,Kathryn E Hentges

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB75674)

Western blot - Anti-ALDH1A2 antibody (AB75674)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise