AB96060

Anti-ALDH1A2 antibody

Name: Anti-ALDH1A2 antibody (ab96060) | Abcam
Brand: Abcam Limited
SKU: AB96060
Price: 565 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(14 Publications)

Rabbit Polyclonal ALDH1A2 antibody. Suitable for IHC - Wmt, WB, IHC-P and reacts with Zebrafish, Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A2 aa 200 to C-terminus.

View Alternative Names

RALDH2, ALDH1A2, Retinal dehydrogenase 2, RALDH 2, RalDH2, Aldehyde dehydrogenase family 1 member A2, Retinaldehyde-specific dehydrogenase type 2, RALDH(II)

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB96060)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB96060)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for ALDH1A2 protein at cytoplasm with ab96060 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-ALDH1A2 antibody (AB96060)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilution

Lane 1:

K562 cell lysates at 30 µg

Lane 2:

Jurkat cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-ALDH1A2 antibody (AB96060)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH1A2 antibody (ab96060) at 1/10000 dilution

All lanes:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

IHC - Wholemount - Anti-ALDH1A2 antibody (AB96060)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-ALDH1A2 antibody (AB96060)

ALDH1A2 antibody [C1C3] detects Aldh1a2 protein on whole mount zebrafish by immunohistochemical analysis.
ab96060 was used at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human ALDH1A2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A2 also known as Retinaldehyde Dehydrogenase 2 (RALDH2) is an enzyme that plays an important role in the metabolic conversion of retinaldehyde to retinoic acid a bioactive form of vitamin A. It has a known mass of approximately 56 kDa. ALDH1A2 has high expression in multiple tissues including the liver pancreas and various types of epithelial cells. This enzyme is cytosolic actively contributing to local retinoid metabolism.

Biological function summary

ALDH1A2 is essential in embryonic development and tissue differentiation. It acts by regulating the levels of retinoic acid necessary for directing gene expression. ALDH1A2 activity is not known to be part of a complex but interacts with other retinoid-binding proteins to achieve its functions. It orchestrates cellular processes by modulating growth factors that are sensitive to retinoic acid.

Pathways

ALDH1A2 is a participant in the retinol metabolism pathway and the broader RA-signaling pathway. It ensures the generation of retinoic acid critical for gene regulation within these pathways. RALDH2's activity is closely related to other dehydrogenases and aldehyde oxidases that contribute to retinoid homeostasis. The interplay with these proteins emphasizes its role in ensuring a balanced retinoic acid supply.

ALDH1A2 has been implicated in conditions such as congenital diaphragmatic hernia and certain cancer types like breast cancer. Aberrant regulation or mutations in ALDH1A2 can lead to altered retinoic acid levels affecting cell proliferation and differentiation pathways. Networks involving ALDH1A2 with proteins like CYP26B1 responsible for retinoic acid catabolism highlight its involvement in these medical conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively (PubMed : 29240402, PubMed : 33565183). Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable) (PubMed : 33565183). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein (By similarity). Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal (By similarity). Displays complete lack of activity with citral (By similarity).

See full target information ALDH1A2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 11:1111684 PubMed37261075

2023

Recapitulating porcine cardiac development : from expanded potential stem cell to embryo culture models.

Applications

Unspecified application

Species

Unspecified reactive species

Hilansi Rawat,Jessica Kornherr,Dorota Zawada,Sara Bakhshiyeva,Christian Kupatt,Karl-Ludwig Laugwitz,Andrea Bähr,Tatjana Dorn,Alessandra Moretti,Monika Nowak-Imialek

Frontiers in oncology 12:762820 PubMed35372040

2022

Disulfiram Transcends ALDH Inhibitory Activity When Targeting Ovarian Cancer Tumor-Initiating Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael W Caminear,Brittney S Harrington,Rahul D Kamdar,Michael J Kruhlak,Christina M Annunziata

eLife 10: PubMed34643182

2021

Tbx5 drives expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development.

Applications

Unspecified application

Species

Unspecified reactive species

Scott A Rankin,Jeffrey D Steimle,Xinan H Yang,Ariel B Rydeen,Kunal Agarwal,Praneet Chaturvedi,Kohta Ikegami,Michael J Herriges,Ivan P Moskowitz,Aaron M Zorn

PLoS biology 19:e3001200 PubMed33999917

2021

Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak.

Applications

Unspecified application

Species

Unspecified reactive species

Kenzo Ivanovitch,Pablo Soro-Barrio,Probir Chakravarty,Rebecca A Jones,Donald M Bell,S Neda Mousavy Gharavy,Despina Stamataki,Julien Delile,James C Smith,James Briscoe

Journal of cellular and molecular medicine : PubMed33822475

2021

Establishment and characterization of an immortalized epicardial cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Haobin Jiang,Shen Song,Jiacheng Li,Qianqian Yin,Shengshou Hu,Yu Nie

Development (Cambridge, England) 148: PubMed33298461

2021

Axial skeleton anterior-posterior patterning is regulated through feedback regulation between Meis transcription factors and retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra C López-Delgado,Irene Delgado,Vanessa Cadenas,Fátima Sánchez-Cabo,Miguel Torres

Developmental cell 52:350-363.e6 PubMed31928974

2020

A Second Heart Field-Derived Vasculogenic Niche Contributes to Cardiac Lymphatics.

Applications

Unspecified application

Species

Unspecified reactive species

Ghislaine Lioux,Xiaolei Liu,Susana Temiño,Michael Oxendine,Estefanía Ayala,Sagrario Ortega,Robert G Kelly,Guillermo Oliver,Miguel Torres

Respiratory research 20:292 PubMed31870435

2019

TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Wojahn,Timo H Lüdtke,Vincent M Christoffels,Mark-Oliver Trowe,Andreas Kispert

American journal of physiology. Lung cellular and 316:L767-L783 PubMed30702346

2019

Mesothelial mobilization in the developing lung and heart differs in timing, quantity, and pathway dependency.

Applications

Unspecified application

Species

Unspecified reactive species

Timo H Lüdtke,Carsten Rudat,Jennifer Kurz,Regine Häfner,Franziska Greulich,Irina Wojahn,Nurullah Aydoğdu,Tamrat M Mamo,Marc-Jens Kleppa,Mark-Oliver Trowe,Tobias Bohnenpoll,Makoto Mark Taketo,Andreas Kispert

Journal of cellular biochemistry 119:6882-6893 PubMed29665144

2018

Disulfiram/copper targets stem cell-like ALDH population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Jin,Xiaojian Zhu,Fanjun Cheng,Liling Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (AB96060)

Western blot - Anti-ALDH1A2 antibody (AB96060)

Western blot - Anti-ALDH1A2 antibody (AB96060)

IHC - Wholemount - Anti-ALDH1A2 antibody (AB96060)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise