AB156019

Anti-ALDH1A2 antibody [EPR9370]

Name: Anti-ALDH1A2 antibody [EPR9370] (ab156019) | Abcam
Brand: Abcam Limited
SKU: AB156019
Price: 395 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal ALDH1A2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

RALDH2, ALDH1A2, Retinal dehydrogenase 2, RALDH 2, RalDH2, Aldehyde dehydrogenase family 1 member A2, Retinaldehyde-specific dehydrogenase type 2, RALDH(II)

1 Images

Unknown

Western blot - Anti-ALDH1A2 antibody [EPR9370] (AB156019)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ALDH1A2 antibody [EPR9370] (ab156019) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 2:

Mouse testis lysates at 20 µg

Lane 3:

Rat testis lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Carrier free

Anti-ALDH1A2 antibody [EPR9370] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9370

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A2 also known as Retinaldehyde Dehydrogenase 2 (RALDH2) is an enzyme that plays an important role in the metabolic conversion of retinaldehyde to retinoic acid a bioactive form of vitamin A. It has a known mass of approximately 56 kDa. ALDH1A2 has high expression in multiple tissues including the liver pancreas and various types of epithelial cells. This enzyme is cytosolic actively contributing to local retinoid metabolism.

Biological function summary

ALDH1A2 is essential in embryonic development and tissue differentiation. It acts by regulating the levels of retinoic acid necessary for directing gene expression. ALDH1A2 activity is not known to be part of a complex but interacts with other retinoid-binding proteins to achieve its functions. It orchestrates cellular processes by modulating growth factors that are sensitive to retinoic acid.

Pathways

ALDH1A2 is a participant in the retinol metabolism pathway and the broader RA-signaling pathway. It ensures the generation of retinoic acid critical for gene regulation within these pathways. RALDH2's activity is closely related to other dehydrogenases and aldehyde oxidases that contribute to retinoid homeostasis. The interplay with these proteins emphasizes its role in ensuring a balanced retinoic acid supply.

ALDH1A2 has been implicated in conditions such as congenital diaphragmatic hernia and certain cancer types like breast cancer. Aberrant regulation or mutations in ALDH1A2 can lead to altered retinoic acid levels affecting cell proliferation and differentiation pathways. Networks involving ALDH1A2 with proteins like CYP26B1 responsible for retinoic acid catabolism highlight its involvement in these medical conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively (PubMed : 29240402, PubMed : 33565183). Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable) (PubMed : 33565183). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein (By similarity). Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal (By similarity). Displays complete lack of activity with citral (By similarity).

See full target information ALDH1A2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1277 PubMed36882470

2023

CTCF controls three-dimensional enhancer network underlying the inflammatory response of bone marrow-derived dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bobae Yang,Sueun Kim,Woong-Jae Jung,Kyungwoo Kim,Sugyung Kim,Yong-Jin Kim,Tae-Gyun Kim,Eun-Chong Lee,Jung-Sik Joo,Chae Gyu Park,Sumin Oh,Kyung Hyun Yoo,Hyoung-Pyo Kim

Biology 12: PubMed36671735

2022

Critical Role of Cathepsin L/V in Regulating Endothelial Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Li,Zhaoya Liu,Mengshi Chen,Liyang Zhang,Ruizheng Shi,Hua Zhong

Respiratory research 21:186 PubMed32678044

2020

In vivo miR-138-5p inhibition alleviates monocrotaline-induced pulmonary hypertension and normalizes pulmonary KCNK3 and SLC45A3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Le Ribeuz,Audrey Courboulin,Maria-Rosa Ghigna,Mélanie Lambert,Aurélie Hautefort,Marc Humbert,David Montani,Sylvia Cohen-Kaminsky,Frédéric Perros,Fabrice Antigny

PloS one 12:e0170937 PubMed28129349

2017

A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Yasgar,Steven A Titus,Yuhong Wang,Carina Danchik,Shyh-Ming Yang,Vasilis Vasiliou,Ajit Jadhav,David J Maloney,Anton Simeonov,Natalia J Martinez

EMBO molecular medicine 7:819-30 PubMed25864124

2015

Fusion protein of retinol-binding protein and albumin domain III reduces liver fibrosis.

Applications

Species

Unspecified reactive species

Hongsik Lee,Hyeyeun Jeong,Sangeun Park,Wonbaek Yoo,Soyoung Choi,Kyungmin Choi,Min-Goo Lee,Mihwa Lee,DaeRyong Cha,Young-Sik Kim,Jeeyoung Han,Wonkon Kim,Sun-Hwa Park,Junseo Oh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1A2 antibody [EPR9370]

Western blot - Anti-ALDH1A2 antibody [EPR9370] (AB156019)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise