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AB249258

Anti-ALDH1A2 antibody [EPR9370] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ALDH1A2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RALDH2, ALDH1A2, Retinal dehydrogenase 2, RALDH 2, RalDH2, Aldehyde dehydrogenase family 1 member A2, Retinaldehyde-specific dehydrogenase type 2, RALDH(II)

1 Images
Western blot - Anti-ALDH1A2 antibody [EPR9370] - BSA and Azide free (AB249258)
  • WB

Unknown

Western blot - Anti-ALDH1A2 antibody [EPR9370] - BSA and Azide free (AB249258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156019).

All lanes:

Western blot - Anti-ALDH1A2 antibody [EPR9370] (<a href='/en-us/products/primary-antibodies/aldh1a2-antibody-epr9370-ab156019'>ab156019</a>) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 2:

Mouse testis lysates at 20 µg

Lane 3:

Rat testis lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9370

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab249258 is the carrier-free version of ab156019.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A2 also known as Retinaldehyde Dehydrogenase 2 (RALDH2) is an enzyme that plays an important role in the metabolic conversion of retinaldehyde to retinoic acid a bioactive form of vitamin A. It has a known mass of approximately 56 kDa. ALDH1A2 has high expression in multiple tissues including the liver pancreas and various types of epithelial cells. This enzyme is cytosolic actively contributing to local retinoid metabolism.
Biological function summary

ALDH1A2 is essential in embryonic development and tissue differentiation. It acts by regulating the levels of retinoic acid necessary for directing gene expression. ALDH1A2 activity is not known to be part of a complex but interacts with other retinoid-binding proteins to achieve its functions. It orchestrates cellular processes by modulating growth factors that are sensitive to retinoic acid.

Pathways

ALDH1A2 is a participant in the retinol metabolism pathway and the broader RA-signaling pathway. It ensures the generation of retinoic acid critical for gene regulation within these pathways. RALDH2's activity is closely related to other dehydrogenases and aldehyde oxidases that contribute to retinoid homeostasis. The interplay with these proteins emphasizes its role in ensuring a balanced retinoic acid supply.

ALDH1A2 has been implicated in conditions such as congenital diaphragmatic hernia and certain cancer types like breast cancer. Aberrant regulation or mutations in ALDH1A2 can lead to altered retinoic acid levels affecting cell proliferation and differentiation pathways. Networks involving ALDH1A2 with proteins like CYP26B1 responsible for retinoic acid catabolism highlight its involvement in these medical conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively (PubMed : 29240402, PubMed : 33565183). Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable) (PubMed : 33565183). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein (By similarity). Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal (By similarity). Displays complete lack of activity with citral (By similarity).
See full target information ALDH1A2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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