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AB129815

Anti-ALDH1A3 antibody

4

(7 Reviews)

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(46 Publications)

Anti-ALDH1A3 antibody (ab129815) is a rabbit polyclonal antibody detecting ALDH1A3 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 30 publications

View Alternative Names

ALDH6, ALDH1A3, Retinaldehyde dehydrogenase 3, RALDH-3, RalDH3, Aldehyde dehydrogenase 6, Aldehyde dehydrogenase family 1 member A3

3 Images
Western blot - Anti-ALDH1A3 antibody (AB129815)
  • WB

Unknown

Western blot - Anti-ALDH1A3 antibody (AB129815)

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129815 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-ALDH1A3 antibody (ab129815) at 1 µg/mL

Lane 1:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Lane 2:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 3:

Kidney (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 12 kDa,32 kDa,37 kDa,47 kDa,50 kDa,56 kDa

true

Exposure time: 1min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody (AB129815)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody (AB129815)

IHC image of ab129815 staining in human stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129815, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A3 antibody (AB129815)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A3 antibody (AB129815)

ab129815 staining ALDH1A3 in PC3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129815 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of ab129815 are tested in WB. Previous batches were additionally validated in ICC/IF, and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.

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Reactivity data

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"IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Syrian hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-ALDH1A3 antibody (ab129815) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of ALDH1A3?
Anti-ALDH1A3 (ab129815) specifically detects a band for ALDH1A3 (UniProt: P47895) at a molecular weight of 56kDa.

Trusted by the scientific community
Anti-ALDH1A3 (ab129815) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-ALDH1A3 (ab129815) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A3 also known as Aldehyde Dehydrogenase 1 Family Member A3 is an enzyme with a molecular mass of approximately 55 kDa. It belongs to the aldehyde dehydrogenase family which is responsible for oxidizing aldehydes into carboxylic acids. ALDH1A3 plays an important role in the metabolism of retinaldehyde into retinoic acid an important biochemical reaction in the body. This enzyme shows high expression in tissues such as the liver kidney and neural tissue.
Biological function summary

The role of ALDH1A3 extends beyond simple metabolic processes. It is involved in the regulation of retinoid signaling influencing various cellular processes such as differentiation and proliferation. ALDH1A3 does not operate in isolation; it is often part of a complex interplay of enzymes that regulate local concentrations of retinoic acid which serves as a signaling molecule influencing gene expression. This regulatory capability underlines its importance in developmental and physiological patterns.

Pathways

ALDH1A3 is essential in the retinoic acid signaling pathway and the metabolic pathway of vitamin A derivatives. It interacts with other ALDH family members such as ALDH1A1 and ALDH1A2 in the synthesis and degradation of retinoic acid. Through these pathways ALDH1A3 also impacts the Wnt and Hedgehog pathways which are important for cellular growth and differentiation.

ALDH1A3 connects with several neurodegenerative and cancer conditions. Studies have indicated a correlation between ALDH1A3 expression and certain types of cancer including breast cancer and glioblastoma. In cancer aberrant ALDH1A3 levels can affect cancer stem cell properties and tumor progression. Additionally the protein interacts with other proteins like ALDH1L1 and ALDH3A1 which might also play roles in tumor metabolism and resistance to chemotherapy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively (By similarity) (PubMed : 27759097). High specificity for all-trans-retinal as substrate, can also accept acetaldehyde as substrate in vitro but with lower affinity (PubMed : 27759097). Required for the biosynthesis of normal levels of retinoate in the embryonic ocular and nasal regions; a critical lipid in the embryonic development of the eye and the nasal region (By similarity).
See full target information ALDH1A3
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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Cell and tissue research 401:59-68 PubMed40323407

2025

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Steingruber,Simona Handtke,Franziska Schweiger,Stefan Schiele,Bruno Märkl,Marco Koch

Cell reports 43:114406 PubMed38963759

2024

ALDH1A3-acetaldehyde metabolism potentiates transcriptional heterogeneity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Lu,Jana Travnickova,Mihaly Badonyi,Florian Rambow,Andrea Coates,Zaid Khan,Jair Marques,Laura C Murphy,Pablo Garcia-Martinez,Richard Marais,Pakavarin Louphrasitthiphol,Alex H Y Chan,Christopher J Schofield,Alex von Kriegsheim,Joseph A Marsh,Valeria Pavet,Owen J Sansom,Robert S Illingworth,E Elizabeth Patton

BMC cancer 24:319 PubMed38454443

2024

Regulation of cancer stem cells by CXCL1, a chemokine whose secretion is controlled by MCM2.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Jee Kahm,In-Gyu Kim,Rae-Kwon Kim

Cell and tissue research 394:515-528 PubMed37904003

2023

ALDH1A1 and ALDH1A3 paralogues of aldehyde dehydrogenase 1 control myogenic differentiation of skeletal muscle satellite cells by retinoic acid-dependent and -independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Steingruber,Florian Krabichler,Sophie Franzmeier,Wei Wu,Jürgen Schlegel,Marco Koch

Cells 12: PubMed37947601

2023

Enhanced Sensitivity to ALDH1A3-Dependent Ferroptosis in TMZ-Resistant Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wu,Sophie Franzmeier,Friederike Liesche-Starnecker,Jürgen Schlegel

CNS neuroscience & therapeutics 30:e14396 PubMed37551838

2023

MiR-4524b-5p-targeting ALDH1A3 attenuates the proliferation and radioresistance of glioblastoma via PI3K/AKT/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Yu,Xiaodong Li,Yi Li,Tuo Wang,Maode Wang,Ping Mao

Biomedicines 11: PubMed37239133

2023

Regulation of Cancer Stem Cells and Epithelial-Mesenchymal Transition by CTNNAL1 in Lung Cancer and Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Jee Kahm,Uhee Jung,Rae-Kwon Kim

International journal of molecular sciences 24: PubMed37047826

2023

RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Jee Kahm,In-Gyu Kim,Rae-Kwon Kim

Aging 15:2347-2357 PubMed36996494

2023

MiR-320b aberrant expression enhances the radioresistance of human glioma via upregulated expression of ALDH1A3.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Mao,Tuo Wang,Ke Gao,Yi Li,Changwang Du,Maode Wang

Current issues in molecular biology 45:2170-2181 PubMed36975509

2023

In Vitro Evaluation of ALDH1A3-Affinic Compounds on Breast and Prostate Cancer Cell Lines as Single Treatments and in Combination with Doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Osama H Abusara,Ali I M Ibrahim,Hamzah Issa,Alaa M Hammad,Worood H Ismail
View all publications
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