AB246928

Anti-ALDH1B1 antibody

Name: Anti-ALDH1B1 antibody (ab246928) | Abcam
Brand: Abcam Limited
SKU: AB246928
Price: 655 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ALDH1B1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1B1 aa 1-100.

View Alternative Names

ALDH5, ALDHX, ALDH1B1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase family 1 member B1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling ALDH1B1 with ab246928 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ALDH1B1 with ab246928 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ALDH1B1 with ab246928 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunocytochemistry - Anti-ALDH1B1 antibody (AB246928)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ALDH1B1 using ab246928 at 4 μg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-ALDH1B1 antibody (AB246928)

All lanes:

Western blot - Anti-ALDH1B1 antibody (ab246928) at 0.04 µg/mL

All lanes:

RH-30 (Human bone marrow metastasis cell line) whole cell lysate

Predicted band size: 57 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALDH1B1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P30837

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme ALDH1B1 also known as aldehyde dehydrogenase 1 family member B1 plays a critical role in converting aldehydes to their corresponding carboxylic acids. ALDH1B1 possesses a molecular mass of approximately 56 kDa. This enzyme is expressed in various tissues with particular abundance in the liver and the gastrointestinal tract where it facilitates the detoxification and metabolism of aldehydes.

Biological function summary

This enzyme participates in the oxidation of aldehydes derived from alcohol metabolism enhancing cellular resilience by minimizing toxic aldehyde accumulation. ALDH1B1 occasionally acts as part of larger enzyme complexes where it collaborates with other members of the aldehyde dehydrogenase family to maintain cellular homeostasis and aid in metabolic processes. The enzyme ensures efficient functioning of aldehyde detoxification systems contributing to cellular health.

Pathways

This enzyme is integral to the ethanol metabolism pathway and the one-carbon metabolism pathway. In the ethanol metabolism pathway ALDH1B1 complements other aldehyde dehydrogenase enzymes like ALDH2 converting acetaldehyde to acetate and facilitating safe excretion. In one-carbon metabolism ALDH1B1 influences folate cycle components potentially impacting methylation processes and folate-dependent synthesis pathways. These pathways highlight the enzyme’s role in protecting cells from aldehyde-induced toxicity and maintaining metabolic balance.

ALDH1B1 has been linked to alcohol-related liver disease where its dysfunction may impair acetaldehyde clearance contributing to tissue damage and inflammation. Furthermore abnormal ALDH1B1 activity is associated with colorectal cancer implicating disrupted aldehyde detoxification in tumorigenesis. The enzyme's interaction with proteins such as ALDH2 in these conditions highlights its importance in regulating cellular and tissue health in response to metabolic and alcohol-induced stresses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation.

See full target information ALDH1B1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 8:175 PubMed37121942

2023

Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ziruoyu Wang,Yanan Li,Wensi Zhao,Shuai Jiang,Yuqi Huang,Jun Hou,Xuelu Zhang,Zhaoyu Zhai,Chen Yang,Jiaqi Wang,Jiying Zhu,Jianbo Pan,Wei Jiang,Zengxia Li,Mingliang Ye,Minjia Tan,Haowen Jiang,Yongjun Dang

Histology and histopathology 36:183-194 PubMed33438176

2021

Aldehyde dehydrogenase 1B1 is a potential marker of colorectal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Hejing Wang,Yanmeng Li,Donghu Zhou,Xiaojin Li,Siyu Jia,Saiping Qi,Jian Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1B1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1B1 antibody (AB246928)

Immunocytochemistry - Anti-ALDH1B1 antibody (AB246928)

Western blot - Anti-ALDH1B1 antibody (AB246928)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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