Rabbit Recombinant Monoclonal ALDH1L1 antibody. Astrocyte marker. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44999.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463)")
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img103413.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463)")
![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-frozen-sections-img96853.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463)")
![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-frozen-sections-img114947.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71065.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (AB177463)")
Publications
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- Bioengineered 13:1952-19612022Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78.
- Acta neuropathologica communications 10:722022Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaquel N Taddei,Maria V Sanchez-Mico,Orla Bonnar,Theresa Connors,Angelica Gaona,Dominique Denbow,Matthew P Frosch,Teresa Gómez-IslaPubMed 35534858
- Neurophotonics 9:0219032022Overview of extracellular vesicle characterization techniques and introduction to combined reflectance and fluorescence confocal microscopy to distinguish extracellular vesicle subpopulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCanan Bağcı,Melike Sever-Bahcekapili,Nevin Belder,Adam P S Bennett,Şefik Evren Erdener,Turgay DalkaraPubMed 35386596
- Oncology reports 46:2021RORβ suppresses the stemness of gastric cancer cells by downregulating the activity of the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Wen,Ming Chen,Wenhao Guo,Ke Guo,Ping Du,Yanfei Fang,Min Gao,Qiang WangPubMed 34278502
- Brain structure & function 225:639-6672020Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpad Dobolyi et. al.PubMed 31982949
- Nature communications 10:41122019In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCyril J Peter et. al.PubMed 31511512
- Frontiers in neural circuits 13:552019Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiedre Milinkeviciute et. al.PubMed 31555101
- Australian veterinary journal 97:277-2822019Large felid leucoencephalomyelopathy in a Sumatran tiger (Panthera tigris sumatrae) from an Australian zoo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD M Hanshaw et. al.PubMed 31209862
- International journal of developmental neuroscienc 42:24-362015Transcriptional analysis of glial cell differentiation in the postnatal murine spinal cord.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Expected |
| Mouse | Expected | Not recommended | Expected | Not recommended | Tested |
| Rat | Expected | Not recommended | Expected | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
Additional Targets
Mitochondrial 10-formyltetrahydrofolate dehydrogenase
Alternative names
FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1
Recommended products
Rabbit Recombinant Monoclonal ALDH1L1 antibody. Astrocyte marker. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12743(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH1L1 and ALDH1L2 also called 10-formyltetrahydrofolate dehydrogenases are enzymes involved in folate metabolism. The enzymes have molecular masses of approximately 110 kDa. They are predominantly expressed in astrocytes in the central nervous system. ALDH1L1 is widely recognized as an astrocyte marker due to its specific expression pattern in these glial cells in the brain. The enzyme plays a role in one-carbon metabolism necessary for synthesizing nucleotides and other cellular processes.
- Biological function summary
ALDH1L1 and ALDH1L2 participate in the conversion of 10-formyltetrahydrofolate to tetrahydrofolate. This reaction is important for the regulation of cellular one-carbon units. They function as part of the folate metabolic pathway often interacting with proteins like methylene tetrahydrofolate reductase. As an important regulator in folate metabolism ALDH1L1 particularly serves as an astrocyte marker which helps in identifying these cells within the brain tissue.
- Pathways
ALDH1L1 and ALDH1L2 are important components of the folate and methionine cycles. These cycles play a significant role in cell growth and proliferation. Within the methionine cycle ALDH1L1 interacts with proteins like methionine synthase facilitating methionine regeneration from homocysteine. This connection highlights the enzymes' integrative role in maintaining cellular methylation and genetic expression through their metabolic functions.
- Associated diseases and disorders
Disturbances in ALDH1L1 and ALDH1L2 activity can link to neurological conditions such as Alzheimer's disease and epilepsy. ALDH1L1 as an astrocyte marker is often researched in the context of neuroinflammation and neurodegeneration. Dysregulation in folate metabolism may affect homocysteine levels potentially connecting ALDH1L1 with proteins such as S-adenosylhomocysteine hydrolase in these diseases. Understanding these enzymes' role in disease pathways offers potential therapeutic targets for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44999.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img103413.jpg "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
All lanes: Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1: HepG2 lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: Fetal brain lysate at 10 µg
Lane 4: Fetal kidney lysate at 10 µg
Lane 5: Fetal liver lysate at 10 µg
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa
![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-frozen-sections-img96853.jpg "Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Cytoplasmic staining on mouse liver.![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-frozen-sections-img114947.jpg "Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Cytoplasmic staining on rat liver.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71065.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img57197.jpg "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
All lanes: Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1: Fetal liver lysate at 10 µg
Lane 2: 1X PBS (negative control)
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img421557.jpg "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Lanes 1 - 2: Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - BSA and Azide free (Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - BSA and Azide free ab233016)
Lane 1: His-tagged mouse ALDH1L1 fragment recombinant protein (aa162-366) at 10 ng
Lane 2: His-tagged mouse ALDH1L2 fragment recombinant protein (aa184-387) at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25 kDa
Exposure time: 20s
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img57197.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
![Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-aldh1l2-antibody-epr12743b-astrocyte-marker-ab177463--western-blot-img421557.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
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