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Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] (AB231018), expandable thumbnail
  • Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail
  • Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ALDH1L1. Database link O75891

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Rat
Tested
Expected
Expected
African green monkey
Tested
Expected
Expected
Dog
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/500 - 1/2000
Notes

-

Species
African green monkey
Dilution info
1/500 - 1/2000
Notes

-

Species
Rat
Dilution info
1/500 - 1/2000
Notes

-

Expected
Expected

Species
Dog
Dilution info
1/500 - 1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
10 µg/mL
Notes

-

Species
Dog
Dilution info
10 µg/mL
Notes

-

Species
Rat
Dilution info
10 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
1/100
Notes

-

Species
Dog
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

Alternative names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

Recommended products

Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human ALDH1L1. Database link O75891
Clone number
2A6
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.

Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

Associated diseases and disorders

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)

    ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-ALDH1L1 antibody

    Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] (ab231018), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] (ab231018)

    Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

  • Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail

    Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)

    ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody

    All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution

    Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

    Lane 2: pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 99 kDa

  • Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail

    Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)

    ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody

    All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

    Lane 3: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

    Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 99 kDa

  • Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)

    ALDH1L1 Flow Cytometry (Intracellular) staining using mouse Anti-ALDH1L1 antibody

    Intracellular flow cytometric analysis ofJurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with anon-specific negative control antibody (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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