Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
WB | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Rat | Tested | Expected | Expected |
African green monkey | Tested | Expected | Expected |
Dog | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species African green monkey | Dilution info 1/500 - 1/2000 | Notes - |
Species Rat | Dilution info 1/500 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dog | Dilution info 1/500 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 10 µg/mL | Notes - |
Species Dog | Dilution info 10 µg/mL | Notes - |
Species Rat | Dilution info 10 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/100 | Notes - |
Species Dog | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
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Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1
Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Purified from TCS.
ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.
ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.
ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-ALDH1L1 antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody
All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution
Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Lane 2: pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody
All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg
Lane 3: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg
Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
ALDH1L1 Flow Cytometry (Intracellular) staining using mouse Anti-ALDH1L1 antibody
Intracellular flow cytometric analysis ofJurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with anon-specific negative control antibody (blue).
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