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AB231018

Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker

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(1 Review)

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(1 Publication)

Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.

View Alternative Names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

5 Images
Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)

Intracellular flow cytometric analysis ofJurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with anon-specific negative control antibody (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)

Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)

Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)
  • WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)

All lanes:

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 99 kDa

true

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)
  • WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)

All lanes:

Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate at 5 µg

Predicted band size: 99 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A6

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Dog, Human, African green monkey

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ALDH1L1.

O75891

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
See full target information ALDH1L1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 3: PubMed32238456

2020

The CCR4-NOT complex maintains liver homeostasis through mRNA deadenylation.

Applications

Unspecified application

Species

Unspecified reactive species

Akinori Takahashi,Toru Suzuki,Shou Soeda,Shohei Takaoka,Shungo Kobori,Tomokazu Yamaguchi,Haytham Mohamed Aly Mohamed,Akiko Yanagiya,Takaya Abe,Mayo Shigeta,Yasuhide Furuta,Keiji Kuba,Tadashi Yamamoto
View all publications
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