Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105941.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119365.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)")
![Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--western-blot-img54144.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)")
![Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--western-blot-img34599.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)")
![Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--flow-cytometry-intracellular-img57994.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (AB231018)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ALDH1L1. Database link O75891
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Tested | Expected | Expected |
| African green monkey | Tested | Expected | Expected |
| Dog | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species African green monkey | Dilution info 1/500 - 1/2000 | Notes - |
Species Rat | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info 1/500 - 1/2000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 10 µg/mL | Notes - |
Species Dog | Dilution info 10 µg/mL | Notes - |
Species Rat | Dilution info 10 µg/mL | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/100 | Notes - |
Species Dog | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
Alternative names
FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1
Recommended products
Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, African green monkey, Rat, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDH1L1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ALDH1L1. Database link O75891
- Clone number
- 2A6
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
- Biological function summary
ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.
- Pathways
ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.
- Associated diseases and disorders
ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105941.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-ALDH1L1 antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119365.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-ALDH1L1 antibody
Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
![Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--western-blot-img54144.jpg "Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)")
Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)
ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody
All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution
Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Lane 2: pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
![Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--western-blot-img34599.jpg "Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)")
Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)
ALDH1L1 Western blot staining using mouse Anti-ALDH1L1 antibody
All lanes: Western blot - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg
Lane 3: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg
Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
![Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018), expandable thumbnail](https://content.abcam.com/products/images/aldh1l1-antibody-2a6-astrocyte-marker-ab231018--flow-cytometry-intracellular-img57994.jpg "Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)")
Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [2A6] - Astrocyte Marker (ab231018)
ALDH1L1 Flow Cytometry (Intracellular) staining using mouse Anti-ALDH1L1 antibody
Intracellular flow cytometric analysis ofJurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with anon-specific negative control antibody (blue).
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