JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277623

Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal ALDH1L1 antibody. Suitable for WB, ICC and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1L1 aa 400 to C-terminus.

View Alternative Names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

2 Images
Immunocytochemistry - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (AB277623)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (AB277623)

Cortical neuron-glial cell culture from E20 rat stained for ALDH1L1 (red) using ab277623 at 1/1000 dilution and counter-stained with chicken pAb to GFAP (green) at 1/5000 dilution in Immunocytochemistry. The blue is Hoechst is staining nuclear DNA. ab277623 produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and therefore appear yellow.

Western blot - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (AB277623)
  • WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (AB277623)

Lane 1:

Western blot - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (ab277623)

Lanes 2 - 11:

Western blot - Anti-ALDH1L1 antibody [2E7] - Astrocyte Marker (ab277623) at 1/5000 dilution

Lane 2:

Rat heart tissue

Lane 3:

Rat liver tissue

Lane 4:

Rat kidney tissue

Lane 5:

Rat lung tissue

Lane 6:

Rat brain tissue

Lane 7:

Rat spinal cord tissue

Lane 8:

Mouse brain tissue

Lane 9:

Mouse spinal cord tissue

Lane 10:

NIH/3T3 cell lysate

Lane 11:

HEK-293 cell lysate

Predicted band size: 99 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2E7

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human ALDH1L1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75891

style
left-column

Complementary Products

Assay Kits

AB239705

Dihydrofolate Reductase Assay Kit (Colorimetric)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

  • 0
  • 0
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
Primary Antibodies

AB52642

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642)

  • 4
  • 37
Primary Antibodies

AB181036

Anti-EAAT1 antibody [EPR12686]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (AB181036)

  • 5
  • 3
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/1000", "ICC-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from Tissue Culture supernatant.
Storage buffer
Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
See full target information ALDH1L1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:271 PubMed40774956

2025

Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Maolin Li,Zhifei Qiao,Jizheng Li,Hongli Zhou,Dong Huang,Yan Cai,Xiaolong Li,Zuo Zhang,Jianyun Zhou,Jiyin Zhou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com