Anti-ALDH1L1 antibody [4A12] - Astrocyte Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody [4A12] - Astrocyte Marker (AB315197)

Immunofluorescent analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with ab315197, dilution 1 : 1,000 in green, and costained with chicken pAb to vimentin, CPCA-Vim, dilution 1 : 5,000 in red. The blue is DAPI staining of nuclear DNA. ab315197 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the CPCA-Vim antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing asrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [4A12] - Astrocyte Marker (AB315197)

Western blot analysis of rat and mouse tissue lysates using ab315197, dilution 1 : 5,000 in green : [1] protein standard (red), rat tissue lysates : [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates : [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.

All lanes:

Western blot - Anti-ALDH1L1 antibody [4A12] - Astrocyte Marker (ab315197) at 1/5000 dilution

Lane 2:

Rat heart tissue lysate

Lane 3:

Rat liver tissue lysate

Lane 4:

Rat kidney tissue lysate

Lane 5:

Rat lung tissue lysate

Lane 6:

Rat brain tissue lysate

Lane 7:

Rat spinal cord tissue lysate

Lane 8:

Mouse brain tissue lysate

Lane 9:

Mouse spinal cord tissue lysate

Observed band size: 100 kDa

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