AB226954

Anti-ALDH1L1 antibody - Astrocyte Marker

Name: Anti-ALDH1L1 antibody - Astrocyte Marker (ab226954) | Abcam
Brand: Abcam Limited
SKU: AB226954
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal ALDH1L1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1L1.

View Alternative Names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

4 Images

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab226954) at 1/1000 dilution

All lanes:

Human brain tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab226954) at 1/500 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 99 kDa

true

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab226954) at 1/5000 dilution

All lanes:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 99 kDa

true

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab226954) at 1/5000 dilution

All lanes:

Rat kidney tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ALDH1L1. The exact immunogen used to generate this antibody is proprietary information.

O75891

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.

Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

See full target information ALDH1L1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23470 PubMed37477183

2023

Circ_0058063 regulates cell vitality and proliferation in oesophageal squamous-cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Yang,Bing Zhu,Zhaofeng Ning,Xiaodong Wang,Zhaoxia Li,Chunxia Zhang,Linchun Wen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1L1 antibody - Astrocyte Marker

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB226954)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise