Anti-ALDH1L1 antibody - Astrocyte Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

100% methanol-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).

Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

A) DAPI
B) ab235197 + secondary.
C) Merged image
D) Secondary only control.

Expected localization : Cytoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

Immunohistochemical analysis of mouse liver tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.

Pathologist analysis : ALDH1L1 showed selective cytoplasmic staining in mouse liver of hepatocytes. The specificity of the staining is weak in bile duct cells and strong membranous and cytoplasmic in hepatocytes which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1 : 200.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

Immunohistochemical analysis of mouse kidney tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.

Pathologist analysis : ALDH1L1 showed selective staining of renal tubular epithelial cells in mouse kidney. The specificity of the staining is cytoplasmic and membranous in tubules not in glomeruli which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1 : 200.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).

Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

A) DAPI
B) ab235197 + secondary.
C) Merged image
D) Secondary only control.

Expected localization : Cytoplasm.

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

ALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab235197) at 1/40000 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 99 kDa

Observed band size: 34 kDa,80 kDa

false

Exposure time: 35s

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

Lanes 1 - 4:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab235197) at 1/1000 dilution

Lane 5:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab235197) at 1/40000 dilution

Lane 2:

Recombinant mouse ALDH1L1 protein

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 4:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 5:

Mouse liver (normal tissue) lysate

Secondary

All lanes:

F(ab)'2 Dk-a-Gt IgG [H&L] at 1/40000 dilution

Predicted band size: 99 kDa

Observed band size: 98.7 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB235197)

ALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).

All lanes:

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab235197) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

ALDH1L1 HEK293T overexpressed lysate

Secondary

All lanes:

605-703-125 GOAT IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed at 1/40000 dilution

Predicted band size: 99 kDa

false

Exposure time: 1s