AB175198

Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate labeling ALDH1L1 using ab175198 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (ab175198)

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

All lanes:

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (ab175198) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Predicted band size: 99 kDa

false

Carrier free

Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12744(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.

Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

See full target information ALDH1L1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed33918472

2021

Is ALDH1L1 Elevated in Lung Cancer? Comment on: Lee, S.-H.; et al. "The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer" 2020, , 1382.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey A Krupenko,Jaspreet Sharma

Theranostics 11:3472-3488 PubMed33537098

2021

Overall survival of pancreatic ductal adenocarcinoma is doubled by deletion in the KPC mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Seon Lee,Ho Lee,Sang Myung Woo,Hyonchol Jang,Yoon Jeon,Hee Yeon Kim,Jaewhan Song,Woo Jin Lee,Eun Kyung Hong,Sang-Jae Park,Sung-Sik Han,Soo-Youl Kim

Cancers 12: PubMed32481524

2020

The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in -Driven Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Seon-Hyeong Lee,Yoon Jeon,Joon Hee Kang,Hyonchol Jang,Ho Lee,Soo-Youl Kim

Scientific reports 9:16313 PubMed31705020

2019

Gastric cancer depends on aldehyde dehydrogenase 3A1 for fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Seon Lee,Seung Hwa Kim,Soohyun Lee,Joon Hee Kang,Seon-Hyeong Lee,Jae-Ho Cheong,Soo-Youl Kim

Oncotarget 7:49397-49410 PubMed27384481

2016

Aldehyde dehydrogenase inhibition combined with phenformin treatment reversed NSCLC through ATP depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Joon Hee Kang,Seon-Hyeong Lee,Jae-Seon Lee,Boas Nam,Tae Wha Seong,Jaekyoung Son,Hyonchol Jang,Kyeong Man Hong,Cheolju Lee,Soo-Youl Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (AB175198)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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