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AB249853

Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)

This data was developed using ab175198, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)

This data was developed using ab175198, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)
  • IP

Supplier Data

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)

This data was developed using ab175198, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate labeling ALDH1L1 using ab175198 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (<a href='/en-us/products/primary-antibodies/aldh1l1-antibody-epr12744b-astrocyte-marker-ab175198'>ab175198</a>)

Predicted band size: 99 kDa

false

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)
  • WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - BSA and Azide free (AB249853)

This data was developed using ab175198, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker (<a href='/en-us/products/primary-antibodies/aldh1l1-antibody-epr12744b-astrocyte-marker-ab175198'>ab175198</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Predicted band size: 99 kDa

false

  • Unconjugated

    Anti-ALDH1L1 antibody [EPR12744(B)] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12744(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249853 is the carrier-free version of ab175198.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.
Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed : 19933275, PubMed : 21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
See full target information ALDH1L1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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