Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.
Panel B : anti-ALDH1L1 staining hepatocytes in rat liver.
Panel C : anti-CD31 staining endothelium in rat liver.
Panel D : anti-ABCB11 staining bile canaliculi in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab182981 and ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on astrocytes of mouse cerebrum is observed. The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat liver is observed. The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse liver is observed. The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/250 dilution (1.864 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in mouse primary astrocytes. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 dilution (10 ug/ml), followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307696 at 1/250 dilution followed by ab150120 at 1/1000 dilution. -ve control 2 : ab10062 at 1/50 dilution followed by ab150081 at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/250 dilution (1.864 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in rat primary astrocytes. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 dilution (10 ug/ml), followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307696 at 1/250 dilution followed by ab150120 at 1/1000 dilution. -ve control 2 : ab10062 at 1/50 dilution followed by ab150081 at 1/1000 dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.
Panel B : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel C : anti-CD31 staining endothelium in mouse liver.
Panel D : anti-ABCB11 staining bile canaliculi in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab182981 and ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on astrocytes of rat cerebrum (PMID : 18171944). The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/500 dilution (0.1ug) (Right panel) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/500 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized rat primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/500 dilution (0.1ug) (Right panel) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/500 dilution was used as the secondary antibody.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab322457 anti-PAR1 used at 1/500 dilution and ab315474 anti-ABCB11 used at a 1/2000 dilution.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-PAR1 (magenta; Opal^™690) and anti-ABCB11 (gray; Opal^™570) on mouse liver.
Panel B : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel C : anti-PAR1 staining endothelium in mouse liver.
Panel D : anti-ABCB11 staining bile canaliculi in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab307696, ab322457 and ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab314327 anti-CD73 used at a 1/250 dilution.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on mouse liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in mouse liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in mouse liver.
Panel D : anti-CD73 staining endothelium in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab32572 anti-beta Catenin used at a 1/250 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-beta Catenin (grey; Opal^™570) on mouse liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in mouse liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in mouse liver.
Panel D : anti-beta Catenin staining membrane of hepatocytes in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab32572 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab314327 anti-CD73 used at a 1/250 dilution.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on rat liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in rat liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in rat liver.
Panel D : anti-CD73 staining endothelium in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat liver.
Panel B : anti-COL4A2 staining endothelium in rat liver.
Panel C : anti-ALDH1L1 staining hepatocytes in rat liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse liver.
Panel B : anti-COL4A2 staining endothelium in mouse liver.
Panel C : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab32572 anti-beta Catenin used at a 1/250 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-beta Catenin (grey; Opal^™570) on rat liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in rat liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in rat liver.
Panel D : anti-beta Catenin staining membrane of hepatocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab32572 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : 180 seconds. This antibody does not cross-react with mouse ALDH1L2. In Western blot, anti-ALDH1L1 + ALDH1L2 antibody (ab300509) staining at 1/1000 dilution, anti-His antibody (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696) at 1/1000 dilution

Lane 1:

His-tagged mouse ALDH1L1 fragment at 10 ng

Lane 2:

His-tagged mouse ALDH1L2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696)

Blocking/Dilution buffer : 5% NFDM/TBST. Exposure times : Lane 1 : 10 seconds; Lane 2 : 1 second; Lane 3 : 10 seconds; Lane 4 : 3 seconds. Bands below 100 kDa may due to degradation (PMID : 29979702).

All lanes:

Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false