Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Suitable for mIHC, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB307696), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IHC-Fr	IP
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Tested	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250	Notes -
Species Rat	Dilution info 1/250	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information Aldh1l1

Function

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:31624291). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

Alternative names

Fthfd, Aldh1l1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

Recommended products

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Suitable for mIHC, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25443-103
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH1L1 also known as 10-formyltetrahydrofolate dehydrogenase plays an important role as a metabolic enzyme involved in folate metabolism. Typically ALDH1L1 has a molecular mass of around 100 kDa. You can find this protein expressed strongly in the liver and brain particularly in astrocytes where it serves as a marker for these star-shaped glial cells. The expression of ALDH1L1 indicates its significant presence in these tissues helping researchers identify and study astrocyte function more effectively.

Biological function summary

ALDH1L1 is involved in the one-carbon metabolism pathway contributing to the conversion of formyl groups into CO2. This reaction aids in regulating cellular homeostasis and energy production. Within the biological context ALDH1L1 works independently and does not form a large complex but efficiently interacts with other enzymes involved in folate transformations. Its activity ensures proper folate cycle functioning supporting DNA synthesis and repair.

Pathways

ALDH1L1 importantly integrates into the folate metabolism pathway supporting detoxification and nucleotide biosynthesis. In this pathway ALDH1L1 interacts with the MTHFD1 protein to maintain the balance of one-carbon units necessary for nucleic acid synthesis. Furthermore ALDH1L1 interplays within the methionine cycle influencing the methylation processes important for gene expression and epigenetic regulation.

Associated diseases and disorders

ALDH1L1 has associations with neurological conditions particularly Alzheimer's disease and glioblastoma. Changes in ALDH1L1 expression levels have implication in the progression of these diseases. In the context of glioblastoma researchers observe altered ALDH1L1 expression alongside traditional astrocyte markers like GFAP in the tumor microenvironment. Understanding ALDH1L1's role in these conditions could provide insights for developing therapeutic strategies targeting astrocyte behavior.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse cerebrum tissue using rabbit Anti-ALDH1L1 antibody
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on astrocytes of mouse cerebrum is observed.
The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse liver tissue using rabbit Anti-ALDH1L1 antibody
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on mouse liver is observed.
The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of rat cerebrum using rabbit Anti-ALDH1L1 antibody
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on astrocytes of rat cerebrum (PMID: 18171944).
The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of rat liver tissue using rabbit Anti-ALDH1L1 antibody
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ALDH1L1 with ab307696 at 1/5000 dilution (0.093 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on rat liver is observed.
The section was incubated with ab307696 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cells using rabbit Anti-ALDH1L1 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/250 dilution (1.864 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in mouse primary astrocytes.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 dilution (10 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
-ve control 1: ab307696 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: ab10062 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cells using rabbit Anti-ALDH1L1 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/250 dilution (1.864 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in rat primary astrocytes.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 dilution (10 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
-ve control 1: ab307696 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: ab10062 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Flow Cytometry (Intracellular) staining of mouse primary neural/glia cells using rabbit Anti-ALDH1L1 antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/500 dilution (0.1ug) (Right panel) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/500 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Flow Cytometry (Intracellular) staining of rat primary neural/glia cells using rabbit Anti-ALDH1L1 antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized rat primary neural/glia cells labeling ALDH1L1 with ab307696 at 1/500 dilution (0.1ug) (Right panel) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/500 dilution was used as the secondary antibody.
Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Western blot staining using rabbit Anti-ALDH1L1 antibody
Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1:10 seconds; Lane 2:1 second; Lane 3:10 seconds; Lane 4:3 seconds.

Bands below 100 kDa may due to degradation (PMID: 29979702).
All lanes: Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 99 kDa
Observed band size: 100 kDa
Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Western blot staining using rabbit Anti-ALDH1L1 antibody
Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.

This antibody does not cross-react with mouse ALDH1L2.

In Western blot, anti-ALDH1L1 + ALDH1L2 antibody (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker ab300509) staining at 1/1000 dilution, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696) at 1/1000 dilution
Lane 1: His-tagged mouse ALDH1L1 fragment at 10 ng
Lane 2: His-tagged mouse ALDH1L2 fragment at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Rat liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 anti-HIRA/HIR used at 1/100 dilution and Anti-CD73 antibody [EPR28214-169] ab314327 anti-CD73 used at a 1/250 dilution.
Panel A: merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on rat liver.

Panel B: anti-ALDH1L1 staining cytoplasm of hepatocytes in rat liver.

Panel C: anti-HIRA/HIR staining nucleus of hepatocytes in rat liver.

Panel D: anti-CD73 staining endothelium in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 anti-HIRA/HIR used at 1/100 dilution and Anti-CD73 antibody [EPR28214-169] ab314327 anti-CD73 used at a 1/250 dilution.
Panel A: merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on mouse liver.

Panel B: anti-ALDH1L1 staining cytoplasm of hepatocytes in mouse liver.

Panel C: anti-HIRA/HIR staining nucleus of hepatocytes in mouse liver.

Panel D: anti-CD73 staining endothelium in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Rat liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 anti-HIRA/HIR used at 1/100 dilution and Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 anti-beta Catenin used at a 1/250 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-beta Catenin (grey; Opal^™570) on rat liver.

Panel B: anti-ALDH1L1 staining cytoplasm of hepatocytes in rat liver.

Panel C: anti-HIRA/HIR staining nucleus of hepatocytes in rat liver.

Panel D: anti-beta Catenin staining membrane of hepatocytes in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 and Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 anti-HIRA/HIR used at 1/100 dilution and Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 anti-beta Catenin used at a 1/250 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-beta Catenin (grey; Opal^™570) on mouse liver.

Panel B: anti-ALDH1L1 staining cytoplasm of hepatocytes in mouse liver.

Panel C: anti-HIRA/HIR staining nucleus of hepatocytes in mouse liver.

Panel D: anti-beta Catenin staining membrane of hepatocytes in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-HIRA/HIR antibody [EPR25299-11] ab302928 and Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Rat liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-CD31 antibody [EPR17259] ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in rat liver.

Panel C: anti-CD31 staining endothelium in rat liver.

Panel D: anti-ABCB11 staining bile canaliculi in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-CD31 antibody [EPR17259] ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-CD31 antibody [EPR17259] ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in mouse liver.

Panel C: anti-CD31 staining endothelium in mouse liver.

Panel D: anti-ABCB11 staining bile canaliculi in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307696, Anti-CD31 antibody [EPR17259] ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (ab307696)
ALDH1L1 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-ALDH1L1 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] ab322457 anti-PAR1 used at 1/500 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution.
Panel A: merged staining of anti-ALDH1L1 (green; Opal^™520), anti-PAR1 (magenta; Opal^™690) and anti-ABCB11 (gray; Opal^™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in mouse liver.

Panel C: anti-PAR1 staining endothelium in mouse liver.

Panel D: anti-ABCB11 staining bile canaliculi in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab307696, Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] ab322457 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.