Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Mouse samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (AB300509), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	Dot	WB
Human	Tested	Expected	Tested	Expected	Tested	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Expected	Tested
Rat	Expected	Expected	Tested	Tested	Tested	Expected	Tested
Recombinant full length protein - Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/100	Notes -
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Target data

See full target information ALDH1L1

Function

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

Additional Targets

Mitochondrial 10-formyltetrahydrofolate dehydrogenase, Mitochondrial 10-formyltetrahydrofolate dehydrogenase

Alternative names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25443-54
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH1L1 and ALDH1L2 also known as 10-formyltetrahydrofolate dehydrogenase isoform 1 and 2 are enzymes involved in folate metabolism. ALDH1L1 has a molecular mass of approximately 100 kDa and is prominently expressed in the liver and brain. ALDH1L2 is found mainly in the mitochondria across various tissues. Both enzymes catalyze the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide playing an important role in cellular metabolism.

Biological function summary

The ALDH1L1 and ALDH1L2 participate in the regulation of one-carbon metabolism which is essential for nucleotide biosynthesis and amino acid interconversion. ALDH1L1 functions as part of a complex with other enzymes involved in folate metabolism facilitating the transfer of one-carbon units. These enzymes assist in maintaining proper cellular levels of folate derivatives fundamental for methylation processes and DNA synthesis.

Pathways

ALDH1L1 and ALDH1L2 contribute to the folate cycle and the related methionine cycle. They participate alongside proteins such as methylenetetrahydrofolate reductase (MTHFR) and methionine synthase both of which play roles in homocysteine regulation. Proper functioning of these pathways is necessary for efficient methyl group transfer important for numerous methylation reactions within cells.

Associated diseases and disorders

Impaired function of ALDH1L1 and ALDH1L2 is associated with metabolic disorders including hyperhomocysteinemia and certain types of cancer. Abnormal activity of these enzymes can cause disruptions in folate metabolism leading to elevated homocysteine levels which in turn are linked to cardiovascular diseases. Additionally studies suggest that alterations in folate pathway enzymes including MTHFR might influence cancer progression by affecting DNA methylation status and gene expression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ALDH1L1+ALDH1L2 with ab300509 at 1/5000 (0.107 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on astrocytes of mouse cerebrum. The section was incubated with ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH1L1+ALDH1L2 with ab300509 at 1/5000 (0.107 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse liver. The section was incubated with ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebrum using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ALDH1L1+ALDH1L2 with ab300509 at 1/500 (1.072 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on astrocytes of rat cerebrum (PMID: 18171944). The section was incubated with ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunocytochemistry/ Immunofluorescence staining of HepG2 (human hepatocellular carcinoma epithelial cell) using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling ALDH1L1+ALDH1L2 with ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron/glia cell using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary Astrocyte cells labelling ALDH1L1+ALDH1L2 with ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocyte.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunocytochemistry/ Immunofluorescence staining of rat primary neuron/glia cell using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron/glia cell cells labelling ALDH1L1+ALDH1L2 with ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocyte.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Flow Cytometry (Intracellular) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Flow Cytometry (Intracellular) staining of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell cells labelling ALDH1L1+ALDH1L2 with ab300509 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunohistochemistry (Frozen sections) staining of Mouse cerebrum using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling ALDH1L1+ALDH1L2 with ab300509 at 1/100 (5.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Immunohistochemistry (Frozen sections) staining of Rat cerebrum using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling ALDH1L1+ALDH1L2 with ab300509 at 1/100 (5.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Dot Blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Dot Blot staining using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Dot blot analysis of ALDH1L1 and ALDH1L2 using ab300509 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

Lane1: His-tagged recombinant mouse ALDH1L1 protein

Lane 2: His-tagged recombinant mouse ALDH1L2 protein

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 48 seconds.
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Western blot staining using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with mouse ALDH1L2.
In Western blot, Anti-ALDH1L1+ALDH1L2 antibody (ab300509) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker (Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905) at 1/1000 dilution
Lane 1: His-tagged mouse ALDH1L1 fragment at 10 ng
Lane 2: His-tagged mouse ALDH1L2 fragment at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 24 kDa
Exposure time: 180s
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Western blot staining using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1: 6 seconds; lane 2: 37 seconds; lane 3: 15 seconds; Lane 4: 37 seconds; lanes 5-6: 8 seconds; lane 7: 59 seconds.

Bands below 100 kDa may due to degradation (PMID: 29979702).
All lanes: Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Lane 6: C8-D1A (mouse cerebellum astrocyte) whole cell lysate at 20 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Western blot staining of Human brain tissue lysate using rabbit Anti-ALDH1L1+ALDH1L2 antibody
All lanes: Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/5000 dilution
Observed band size: 100 kDa
Exposure time: 3s
Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab300509 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300509 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse brain tissue lysate 10 ug Lane 2: ab300509 IP in Mouse brain tissue lysate Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300509 in mouse brain tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 32 seconds
Lane 1: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with ab300509 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300509 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human liver tissue lysate 10 ug Lane 2: ab300509 IP in Human liver tissue lysate Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300509 in human liver tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds
Lane 1: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509) at 1/1000 dilution
All lanes: Human liver tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker (ab300509)
ALDH1L1+ALDH1L2 Western blot staining using rabbit Anti-ALDH1L1+ALDH1L2 antibody
Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.

This antibody does not cross-react with mouse ALDH1L2.

In Western blot, anti-ALDH1L1 + ALDH1L2 antibody (ab300509) staining at 1/1000 dilution, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696) at 1/1000 dilution
Lane 1: His-tagged mouse ALDH1L1 fragment at 10 ng
Lane 2: His-tagged mouse ALDH1L2 fragment at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s