Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)

Rabbit Recombinant Monoclonal ALDH1L1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, Dot, WB and reacts with Mouse, Human, Rat, Recombinant full length protein - Mouse samples.

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Images

Dot Blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (AB300510), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	Dot	WB
Human	Tested	Expected	Tested	Expected	Not recommended	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Expected	Tested
Rat	Expected	Expected	Tested	Tested	Tested	Expected	Tested
Recombinant full length protein - Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant full length protein - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info -	Notes -

Target data

See full target information ALDH1L1

Function

Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

Additional Targets

Mitochondrial 10-formyltetrahydrofolate dehydrogenase, Mitochondrial 10-formyltetrahydrofolate dehydrogenase

Alternative names

FTHFD, ALDH1L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25443-54
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot
Storage information: Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH1L1 and ALDH1L2 also known as 10-formyltetrahydrofolate dehydrogenase isoform 1 and 2 are enzymes involved in folate metabolism. ALDH1L1 has a molecular mass of approximately 100 kDa and is prominently expressed in the liver and brain. ALDH1L2 is found mainly in the mitochondria across various tissues. Both enzymes catalyze the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide playing an important role in cellular metabolism.

Biological function summary

The ALDH1L1 and ALDH1L2 participate in the regulation of one-carbon metabolism which is essential for nucleotide biosynthesis and amino acid interconversion. ALDH1L1 functions as part of a complex with other enzymes involved in folate metabolism facilitating the transfer of one-carbon units. These enzymes assist in maintaining proper cellular levels of folate derivatives fundamental for methylation processes and DNA synthesis.

Pathways

ALDH1L1 and ALDH1L2 contribute to the folate cycle and the related methionine cycle. They participate alongside proteins such as methylenetetrahydrofolate reductase (MTHFR) and methionine synthase both of which play roles in homocysteine regulation. Proper functioning of these pathways is necessary for efficient methyl group transfer important for numerous methylation reactions within cells.

Associated diseases and disorders

Impaired function of ALDH1L1 and ALDH1L2 is associated with metabolic disorders including hyperhomocysteinemia and certain types of cancer. Abnormal activity of these enzymes can cause disruptions in folate metabolism leading to elevated homocysteine levels which in turn are linked to cardiovascular diseases. Additionally studies suggest that alterations in folate pathway enzymes including MTHFR might influence cancer progression by affecting DNA methylation status and gene expression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Dot Blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.

Dot blot analysis of ALDH1L1 and ALDH1L2 using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

Lane1: His-tagged recombinant mouse ALDH1L1 protein

Lane 2: His-tagged recombinant mouse ALDH1L2 protein

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 48 seconds.
Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using ab300510, the same antibody clone in a different buffer formulation. ALDH1L1+ALDH1L2 was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human liver tissue lysate 10 ug Lane 2: Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 IP in Human liver tissue lysate Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 in human liver tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds
Lane 1: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/1000 dilution
All lanes: Human liver tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using ab300510, the same antibody clone in a different buffer formulation. ALDH1L1+ALDH1L2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse brain tissue lysate 10 ug Lane 2: Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 IP in Mouse brain tissue lysate Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 in mouse brain tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 32 seconds
Lane 1: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1: 6 seconds; lane 2: 37 seconds; lane 3: 15 seconds; Lane 4: 37 seconds; lanes 5-6: 8 seconds; lane 7: 59 seconds.

Bands below 100 kDa may due to degradation (PMID: 29979702).
All lanes: Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Lane 6: C8-D1A (mouse cerebellum astrocyte) whole cell lysate at 20 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa
Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
All lanes: Western blot - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] (Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509) at 1/5000 dilution
Observed band size: 100 kDa
Exposure time: 3s
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/100 (5.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Flow Cytometry (Intracellular) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell cells labelling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/100 (5.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron/glia cell cells labelling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocyte.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/500 (1.072 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on astrocytes of rat cerebrum (PMID: 18171944). The section was incubated with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/5000 (0.107 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse liver. The section was incubated with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/5000 (0.107 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on astrocytes of mouse cerebrum. The section was incubated with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free (ab300510)
This data was developed using Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron/glia cell cells labelling ALDH1L1+ALDH1L2 with Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] ab300509 at 1/50 (10.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocyte.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.