AB110311

Anti-ALDH2 antibody [9A10AF10]

Name: Anti-ALDH2 antibody [9A10AF10] (ab110311) | Abcam
Brand: Abcam Limited
SKU: AB110311
Price: 430 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal ALDH2 antibody. Suitable for IP, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (AB110311)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (AB110311)

Immunocytochemistry analysis using ab110311 at 5μg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note : Target protein locates mainly in the mitochondrial matrix.

Unknown

Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10] (AB110311)

Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

All lanes:

Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10] (ab110311)

Predicted band size: 56 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9A10AF10

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "For 2 hours." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

ab110311 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.Purity: >95% by SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.

Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.

See full target information ALDH2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 288:34897-905 PubMed24114844

2013

Endoplasmic reticulum-associated degradation controls cell surface expression of γ-aminobutyric acid, type B receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Khaled Zemoura,Marisa Schenkel,Mario A Acuña,Gonzalo E Yévenes,Hanns Ulrich Zeilhofer,Dietmar Benke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH2 antibody [9A10AF10]

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10] (AB110311)

Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10] (AB110311)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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