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AB115198

Anti-ALDH2 antibody

5

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal ALDH2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ALDH2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB115198)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB115198)

ab115198 at 10 µg/ml staining ALDH2 Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Flow Cytometry (Intracellular) - Anti-ALDH2 antibody (AB115198)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ALDH2 antibody (AB115198)

Intracellular flow cytometric analysis of HepG2 cells, staining ALDH2 with ab115198 (bottom histogram), compared to a negative control cell (top histogram). A FITC-conjugated goat-anti-rabbit IgG was used as the secondary antibody.

Western blot - Anti-ALDH2 antibody (AB115198)
  • WB

Unknown

Western blot - Anti-ALDH2 antibody (AB115198)

All lanes:

Western blot - Anti-ALDH2 antibody (ab115198) at 1/50 dilution

Lane 1:

293 cell lysate, non-transfected at 2 µg

Lane 2:

293 cell lysate, transiently transfected with ALDH2 gene at 2 µg

Predicted band size: 56 kDa

Observed band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human ALDH2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P05091

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.
Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
See full target information ALDH2

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7139 PubMed36414669

2022

Cholesterol-induced LRP3 downregulation promotes cartilage degeneration in osteoarthritis by targeting Syndecan-4.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Cao,Yuanyuan Shi,Xin Zhang,Qi Li,Jiahao Zhang,Fengyuan Zhao,Qingyang Meng,Wenli Dai,Zhenlong Liu,Wenqiang Yan,Xiaoning Duan,Jiying Zhang,Xin Fu,Jin Cheng,Xiaoqing Hu,Yingfang Ao

ASN neuro 12:1759091420925977 PubMed32466659

2020

Early Cognitive Impairment Behind Nigrostriatal Circuit Neurotoxicity: Are Astrocytes Involved?

Applications

Unspecified application

Species

Unspecified reactive species

Macarena L Herrera,Romina Deza-Ponzio,Marisa S Ghersi,Emilce A de la Villarmois,Miriam B Virgolini,Mariela F Pérez,Victor A Molina,María J Bellini,Claudia B Hereñú
View all publications

Product promise

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