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AB227021

Anti-ALDH2 antibody

4

(3 Reviews)

|

(9 Publications)

Rabbit Polyclonal ALDH2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Zebrafish, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH2.

View Alternative Names

ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)

Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)

Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (AB227021)

Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ALDH2 antibody (AB227021)
  • WB

Supplier Data

Western blot - Anti-ALDH2 antibody (AB227021)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH2 antibody (ab227021) at 1/500 dilution

Lane 1:

HepG2 whole cell extracts at 30 µg

Lane 2:

HepG2 cytoplasm extracts at 30 µg

Observed band size: 50 kDa

false

Western blot - Anti-ALDH2 antibody (AB227021)
  • WB

Supplier Data

Western blot - Anti-ALDH2 antibody (AB227021)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALDH2 antibody (ab227021) at 1/1000 dilution

Lane 1:

GL261 whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

BCL-1 whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 56 kDa

false

Western blot - Anti-ALDH2 antibody (AB227021)
  • WB

Supplier Data

Western blot - Anti-ALDH2 antibody (AB227021)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH2 antibody (ab227021) at 1/2000 dilution

Lane 1:

Zebrafish liver tissue extracts at 30 µg

Lane 2:

Zebrafish ovary tissue extracts at 30 µg

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALDH2. The exact immunogen used to generate this antibody is proprietary information.

P05091

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>This antibody is recommended to be used with EDTA retrieval solution at pH 8.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>This antibody is recommended to be used with EDTA retrieval solution at pH 8.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.
Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
See full target information ALDH2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1528471 PubMed40313936

2025

CD19 B cell depletion: a novel strategy to alleviate ischemic stroke damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xu,Jing Peng,Yizhong Yan,Min Gao,HongJing Zang,Lamei Cheng,Yu Zhou

Journal of inflammation research 17:10209-10222 PubMed39649425

2024

Comparative Proteomic Analysis of Endolymphatic Sac Luminal Fluid in Patients with Meniere's Disease and Controls.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Qin Wang,Zhou Zhou,Anquan Peng,Wenqi Jiang

Redox biology 76:103318 PubMed39178733

2024

ALDH2 deficiency augments atherosclerosis through the USP14-cGAS-dependent polarization of proinflammatory macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Rui,Huaxiang Yu,Kai Chi,Ziqi Han,Wenyong Zhu,Jian Zhang,Haipeng Guo,Wenyi Zou,Fengxin Wang,Ping Xu,Dan Zou,Xiaoshuai Song,Lulu Liu,Xuting Wu,Wenxiao Wu,Dandan Qin,Yihai Cao,Feng Xu,Li Xue,Yuguo Chen

World journal of gastrointestinal oncology 16:3230-3240 PubMed39072174

2024

Aldehyde dehydrogenase 2 family member repression promotes colorectal cancer progression by JNK/p38 MAPK pathways-mediated apoptosis and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Yu,Qian Chen,Yi-Ping Lu

Food science & nutrition 12:5100-5110 PubMed39055233

2024

Restoring energy metabolism by NAD supplement prevents alcohol-induced liver injury and boosts liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Liu,Cheng Cheng,Han Gao,Xue-Jin Zhu,Xian He,Ming-Xi Zhou,Yuan Gao,Ya-Wen Lu,Xin-Hua Song,Xiao-He Xiao,Jia-Bo Wang,Chun-Jun Xu,Zhi-Tao Ma

Journal of Cancer 15:3724-3737 PubMed38911385

2024

Branched-Chain Amino Acid Degradation Pathway was Inactivated in Colorectal Cancer: Results from a Proteomics Study.

Applications

Unspecified application

Species

Unspecified reactive species

Shixian Lian,Siyuan Liu,Ao Wu,Lin Yin,Lei Li,Liyan Zeng,Mingkun Zhao,Lijun Zhang

The Journal of urology 208:406-413 PubMed35344413

2022

ALDH2 Expression, Alcohol Intake and Semen Parameters among East Asian Men.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel R Greenberg,Hriday P Bhambhvani,Satvir S Basran,Brett P Salazar,Luis Carl Rios,Sin-Jin Li,Che-Hong Chen,Daria Mochly-Rosen,Michael L Eisenberg

Neural regeneration research 17:185-193 PubMed34100455

2021

Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mushfiquddin Khan,Fei Qiao,Pavan Kumar,S M Touhidul Islam,Avtar K Singh,Jeseong Won,Inderjit Singh

Journal of cellular biochemistry 119:6882-6893 PubMed29665144

2018

Disulfiram/copper targets stem cell-like ALDH population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Jin,Xiaojian Zhu,Fanjun Cheng,Liling Zhang
View all publications
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