Rabbit Polyclonal ALDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH2.
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Publications
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- The Journal of urology 208:406-4132022ALDH2 Expression, Alcohol Intake and Semen Parameters among East Asian Men.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel R Greenberg,Hriday P Bhambhvani,Satvir S Basran,Brett P Salazar,Luis Carl Rios,Sin-Jin Li,Che-Hong Chen,Daria Mochly-Rosen,Michael L EisenbergPubMed 35344413
- Journal of cellular biochemistry 119:6882-68932018Disulfiram/copper targets stem cell-like ALDH population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Jin,Xiaojian Zhu,Fanjun Cheng,Liling ZhangPubMed 29665144
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALDH2. The exact immunogen used to generate this antibody is proprietary information. Database link P05091
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Alternative names
ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI
Recommended products
Rabbit Polyclonal ALDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALDH2. The exact immunogen used to generate this antibody is proprietary information. Database link P05091
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.
- Biological function summary
ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.
- Pathways
ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.
- Associated diseases and disorders
ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-ALDH2 antibody (ab227021)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-ALDH2 antibody (ab227021) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody (ab227021)
A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.
Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-ALDH2 antibody (ab227021)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-ALDH2 antibody (ab227021) at 1/1000 dilution
Lane 1: GL261 whole cell lysate at 30 µg
Lane 2: C8D30 whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.
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