AB133306

Anti-ALDH2 antibody [EPR4494]

RabMAb
Recombinant
KO Validated
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(7 Publications)

Rabbit Recombinant Monoclonal ALDH2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI

2 Images

Lab

Western blot - Anti-ALDH2 antibody [EPR4494] (AB133306)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

Lane 1:

Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

ALDH2 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ALDH2 knockout SW480 cell line (<a href='/en-us/products/cell-lines/human-aldh2-knockout-sw480-cell-line-ab270483'>ab270483</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 56 kDa

Observed band size: 53 kDa,56 kDa

false

Unknown

Western blot - Anti-ALDH2 antibody [EPR4494] (AB133306)

All lanes:

Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

Lane 1:

HepG2 lysates at 10 µg

Lane 2:

Human heart lysates at 10 µg

Lane 3:

Fetal liver lysates at 10 µg

Lane 4:

Mouse liver lysates at 10 µg

Lane 5:

Rat liver lysates at 10 µg

Lane 6:

Mouse lung lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4494

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.

Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.

See full target information ALDH2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:11095-11108 PubMed40831518

2025

ALDH2 Ameliorates Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and Pyroptosis by Nrf2/ROS Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuting Tong,Xin Li,Fangying Wang,Qi Cheng,Peiyu Zhang,Mo Chen,Yifan Xie,Xiaoyong Lu,Huaxiang Wu

Chinese medicine 20:83 PubMed40495179

2025

The gut microbiota-mediated ferroptosis pathway: a key mechanism of ginsenoside Rd against metabolism-associated fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liu,Xian Zhou,Liyu Xiao,Xiaolan Huang,Dennis Chang,Xiaomei Zhong,Minjie Zeng,Yanfang Xian,Yanfang Zheng,Wei Huang,Rui Huang,Mingqing Huang

International journal of molecular sciences 25: PubMed39273266

2024

Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution's Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel G Bardallo,Gabriela Chullo,Norma Alva,Joan Rosello-Catafau,Yiliam Fundora-Suárez,Teresa Carbonell,Arnau Panisello-Rosello

Cells 13: PubMed39056755

2024

Astrocytes in Amyloid Generation and Alcohol Metabolism: Implications of Alcohol Use in Neurological Disorder(s).

Applications

Unspecified application

Species

Unspecified reactive species

Mohit Kumar,Natalie Swanson,Sudipta Ray,Shilpa Buch,Viswanathan Saraswathi,Susmita Sil

Cancer biology & therapy 24:2235768 PubMed37548553

2023

A feedback loop between lncRNA MALAT1 and DNMT1 promotes triple-negative breast cancer stemness and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Hu,Yuqiong He,Na Luo,Xin Li,Lei Guo,Kejing Zhang

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 26:183-193 PubMed35477546

2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ding,Quanhua Pan,Long Qian,Chuanxian Hu

International journal of molecular sciences 22: PubMed34069402

2021

Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel G Bardallo,Rui Teixeira da Silva,Teresa Carbonell,Emma Folch-Puy,Carlos Palmeira,Joan Roselló-Catafau,Jacques Pirenne,René Adam,Arnau Panisello-Roselló

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH2 antibody [EPR4494]

Western blot - Anti-ALDH2 antibody [EPR4494] (AB133306)

Western blot - Anti-ALDH2 antibody [EPR4494] (AB133306)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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Product promise