JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB129022

Anti-ALDH3A1 antibody [EPR7406]

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Recombinant Monoclonal ALDH3A1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

ALDH3, ALDH3A1, ALDHIII, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase family 3 member A1

2 Images
Western blot - Anti-ALDH3A1 antibody [EPR7406] (AB129022)
  • WB

Unknown

Western blot - Anti-ALDH3A1 antibody [EPR7406] (AB129022)

All lanes:

Western blot - Anti-ALDH3A1 antibody [EPR7406] (ab129022) at 1/1000 dilution

Lane 1:

A549 cell lysates at 10 µg

Lane 2:

Mouse eyeball lysates at 10 µg

Lane 3:

Rat eyeball lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-abbit at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

OI-RD Scanning - Anti-ALDH3A1 antibody [EPR7406] (AB129022)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ALDH3A1 antibody [EPR7406] (AB129022)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7406

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH3A1 also known as Aldehyde Dehydrogenase 3 Family Member A1 is an enzyme that catalyzes the oxidation of aldehydes to their corresponding carboxylic acids. This enzyme is part of the aldehyde dehydrogenase family and has a molecular mass of approximately 54 kDa. ALDH3A1 is expressed in various tissues notably in the cornea where it plays a protective role against oxidative stress by metabolizing toxic aldehydes resulting from lipid peroxidation and ultraviolet (UV) exposure.
Biological function summary

ALDH3A1 functions in detoxifying harmful aldehydes a process essential for maintaining cellular homeostasis. This enzyme participates in the metabolism of both endogenous and exogenous aldehydes contributing to the cellular defense mechanism. While it does not form part of a known complex its activity ensures reduced reactive aldehyde levels therefore supporting cellular resilience under stress conditions. It holds particular importance in maintaining the transparency and viability of corneal tissues due to its antioxidant properties.

Pathways

ALDH3A1 is involved in metabolic pathways notably in the detoxification pathway where it assists in reducing the burden of reactive aldehyde species. Additionally it plays a role in the alcohol metabolism pathway where it aids in converting acetaldehyde a toxic byproduct of ethanol oxidation into acetic acid. In these pathways ALDH3A1 functions in coordination with other aldehyde dehydrogenases such as ALDH1A1 and ALDH2 providing a comprehensive defense against aldehyde toxicity.

ALDH3A1 has been linked to conditions such as corneal degenerations and alcohol-related liver diseases. Its protective mechanism against oxidative stress is important in preventing corneal opacities and maintaining visual clarity. Furthermore in alcohol metabolism ALDH3A1 complements ALDH2's role mitigating the accumulation of acetaldehyde which is implicated in liver damage and hangover symptoms. Therefore alterations in ALDH3A1 activity can have implications for disease states related to oxidative stress and alcohol metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed : 1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed : 1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).
See full target information ALDH3A1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70236 PubMed39652089

2024

Corneal strain influences keratocyte proliferation and migration through upregulation of ALDH3A1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Xin Zhou,Wei Zhang,Xiaolei Wang,Shengqian Dou,Leilei Zhao,Roine El-Habta,Qingjun Zhou,Ludvig J Backman,Patrik Danielson

Frontiers in oncology 11:659720 PubMed33842376

2021

Jiyuan Oridonin A Overcomes Differentiation Blockade in Acute Myeloid Leukemia Cells With MLL Rearrangements Multiple Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Qu,Yu Duan,Min Zhao,Zhanju Wang,Mengjie Zhao,Yao Zhao,Haihua Wang,Yu Ke,Ying Liu,Hong-Min Liu,Liuya Wei,Zhenbo Hu

Frontiers in bioengineering and biotechnology 8:612091 PubMed33415100

2020

Proteomic Reveals Reasons for Acquired Drug Resistance in Lung Cancer Derived Brain Metastasis Based on a Newly Established Multi-Organ Microfluidic Chip Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxin Xu,Yingyan Wang,Wenzhe Duan,Shengkai Xia,Song Wei,Wenwen Liu,Qi Wang

Antioxidants (Basel, Switzerland) 6: PubMed28671577

2017

Silencing of NRF2 Reduces the Expression of ALDH1A1 and ALDH3A1 and Sensitizes to 5-FU in Pancreatic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Quan Duong,Kyu Sic You,Seunghoon Oh,Sahng-June Kwak,Yeon-Sun Seong

PloS one 12:e0170937 PubMed28129349

2017

A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Yasgar,Steven A Titus,Yuhong Wang,Carina Danchik,Shyh-Ming Yang,Vasilis Vasiliou,Ajit Jadhav,David J Maloney,Anton Simeonov,Natalia J Martinez

Journal of proteome research 14:1535-46 PubMed25599653

2015

Differential reprogramming of isogenic colorectal cancer cells by distinct activating KRAS mutations.

Applications

WB

Species

Human

Dean E Hammond,Craig J Mageean,Emma V Rusilowicz,Julie A Wickenden,Michael J Clague,Ian A Prior
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB103895

Anti-ALDH3A1 antibody

Western blot - Anti-ALDH3A1 antibody (AB103895)

  • 0
  • 0
Primary Antibodies

AB76976

Anti-ALDH3A1 antibody

Western blot - Anti-ALDH3A1 antibody (AB76976)

  • 0
  • 0
Primary Antibodies

AB128902

Anti-ALDH3A1 antibody [EPR7405]

RabMAb|Recombinant

Western blot - Anti-ALDH3A1 antibody [EPR7405] (AB128902)

  • 0
  • 0
Primary Antibodies

AB272015

Anti-ALDH3A1 antibody [SP298] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

  • 0
  • 0
Primary Antibodies

AB227694

Anti-ALDH3A1 antibody [SP298] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (AB227694)

  • 0
  • 0

Complementary Products

Assay Kits

AB155893

ALDH Activity Assay Kit (Colorimetric)

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB168348

Anti-Lumican antibody [EPR8898(2)]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (AB168348)

  • 5
  • 16
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com