JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272015

Anti-ALDH3A1 antibody [SP298] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ALDH3A1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

ALDH3, ALDH3A1, ALDHIII, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase family 3 member A1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human skin tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human stomach tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human cervix tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (AB272015)

Formalin-fixed, paraffin-embedded human bladder tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP298

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB76976

Anti-ALDH3A1 antibody

Western blot - Anti-ALDH3A1 antibody (AB76976)

  • 0
  • 0
Primary Antibodies

AB128902

Anti-ALDH3A1 antibody [EPR7405]

RabMAb|Recombinant

Western blot - Anti-ALDH3A1 antibody [EPR7405] (AB128902)

  • 0
  • 0
Primary Antibodies

AB129022

Anti-ALDH3A1 antibody [EPR7406]

RabMAb|Recombinant

Western blot - Anti-ALDH3A1 antibody [EPR7406] (AB129022)

  • 0
  • 0

Complementary Products

Assay Kits

AB155893

ALDH Activity Assay Kit (Colorimetric)

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

  • 5
  • 1
Primary Antibodies

AB269456

Anti-CD33 antibody [EPR23051-101]

BOND RX™ Validated|20ul selling size|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [EPR23051-101] (AB269456)

  • 5
  • 5
Primary Antibodies

AB77528

Anti-YKL-40/CHI3L1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody (AB77528)

  • 4
  • 3
Primary Antibodies

AB178395

Anti-HGF antibody [EPR12230]

RabMAb|Recombinant

Immunoprecipitation - Anti-HGF antibody [EPR12230] (AB178395)

  • 0
  • 0
Primary Antibodies

AB236640

Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.</p>" } } }
style
left-column

Product details

ab272015 is the carrier-free version of ab227694.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH3A1 also known as Aldehyde Dehydrogenase 3 Family Member A1 is an enzyme that catalyzes the oxidation of aldehydes to their corresponding carboxylic acids. This enzyme is part of the aldehyde dehydrogenase family and has a molecular mass of approximately 54 kDa. ALDH3A1 is expressed in various tissues notably in the cornea where it plays a protective role against oxidative stress by metabolizing toxic aldehydes resulting from lipid peroxidation and ultraviolet (UV) exposure.
Biological function summary

ALDH3A1 functions in detoxifying harmful aldehydes a process essential for maintaining cellular homeostasis. This enzyme participates in the metabolism of both endogenous and exogenous aldehydes contributing to the cellular defense mechanism. While it does not form part of a known complex its activity ensures reduced reactive aldehyde levels therefore supporting cellular resilience under stress conditions. It holds particular importance in maintaining the transparency and viability of corneal tissues due to its antioxidant properties.

Pathways

ALDH3A1 is involved in metabolic pathways notably in the detoxification pathway where it assists in reducing the burden of reactive aldehyde species. Additionally it plays a role in the alcohol metabolism pathway where it aids in converting acetaldehyde a toxic byproduct of ethanol oxidation into acetic acid. In these pathways ALDH3A1 functions in coordination with other aldehyde dehydrogenases such as ALDH1A1 and ALDH2 providing a comprehensive defense against aldehyde toxicity.

ALDH3A1 has been linked to conditions such as corneal degenerations and alcohol-related liver diseases. Its protective mechanism against oxidative stress is important in preventing corneal opacities and maintaining visual clarity. Furthermore in alcohol metabolism ALDH3A1 complements ALDH2's role mitigating the accumulation of acetaldehyde which is implicated in liver damage and hangover symptoms. Therefore alterations in ALDH3A1 activity can have implications for disease states related to oxidative stress and alcohol metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed : 1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed : 1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).
See full target information ALDH3A1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com