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AB250807

Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ALDH4A1/P5CDH antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

ALDH4, P5CDH, ALDH4A1, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling ALDH4A1/P5CDH with ab185208 at 1/160 dilution (red), or a rabbit IgG isotype control (green), followed by secondary goat anti rabbit IgG (FITC) at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of -20°C acetone fixed HeLa cells labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution (red). Secondary antibody : Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi (blue).

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • WB

Supplier Data

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (<a href='/en-us/products/primary-antibodies/aldh4a1-p5cdh-antibody-epr14287-ab185208'>ab185208</a>) at 1/1000 dilution

All lanes:

Human fetal heart at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)
  • WB

Supplier Data

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (AB250807)

This data was developed using ab185208, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (<a href='/en-us/products/primary-antibodies/aldh4a1-p5cdh-antibody-epr14287-ab185208'>ab185208</a>) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14287

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250807 is the carrier-free version of ab185208.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.
Biological function summary

ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.

Pathways

ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.

Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
See full target information ALDH4A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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