AB181256

Anti-ALDH4A1/P5CDH antibody [EPR14288(B)]

RabMAb
Recombinant
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(2 Publications )

Rabbit Recombinant Monoclonal ALDH4A1/P5CDH antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ALDH4, P5CDH, ALDH4A1, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells labeling ALDH4A1/P5CDH with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1/P5CDH, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

Predicted band size: 62 kDa

Observed band size: 62 kDa

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Supplier Data

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

All lanes:

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

true

Supplier Data

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

All lanes:

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

true

Carrier free

Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14288(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.

Biological function summary

ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.

Pathways

ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.

Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

See full target information ALDH4A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13308 PubMed33480139

2021

Incomplete proline catabolism drives premature sperm aging.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-An Yen,Sean P Curran

Nature 589:287-292 PubMed33268892

2020

ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Lorenzo,Pilar Delgado,Christian E Busse,Alejandro Sanz-Bravo,Inmaculada Martos-Folgado,Elena Bonzon-Kulichenko,Alessia Ferrarini,Ileana B Gonzalez-Valdes,Sonia M Mur,Raquel Roldán-Montero,Diego Martinez-Lopez,Jose L Martin-Ventura,Jesús Vázquez,Hedda Wardemann,Almudena R Ramiro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH4A1/P5CDH antibody [EPR14288(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (AB181256)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise