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AB155493

Anti-ALDH5A1/SSADH antibody

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Rabbit Polyclonal ALDH5A1/SSADH antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH5A1 aa 50-350.

View Alternative Names

SSADH, ALDH5A1, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-ALDH5A1/SSADH antibody (AB155493)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ALDH5A1/SSADH antibody (AB155493)

Immunofluorescent analysis of methanol-fixed A431 cells labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution. Lower panel co-stained with Hoescht 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (AB155493)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (AB155493)

Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (AB155493)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (AB155493)

Paraffin-embedded mouse liver tissue labeling ALDH5A1/SSADH using ab155493 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 minutes.

Western blot - Anti-ALDH5A1/SSADH antibody (AB155493)
  • WB

Unknown

Western blot - Anti-ALDH5A1/SSADH antibody (AB155493)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ALDH5A1/SSADH antibody (ab155493) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 57 kDa

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Western blot - Anti-ALDH5A1/SSADH antibody (AB155493)
  • WB

Unknown

Western blot - Anti-ALDH5A1/SSADH antibody (AB155493)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ALDH5A1/SSADH antibody (ab155493) at 1/5000 dilution

All lanes:

Mouse brain whole cell lysate at 20 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALDH5A1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P51649

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigenretrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigenretrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" } } }
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Product details

Keep as a concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH5A1 also known as succinate semialdehyde dehydrogenase (SSADH) functions in the cellular metabolism where it converts succinate semialdehyde to succinate in the presence of NAD+. This enzyme has a molecular weight of approximately 56 kDa and is largely found in the brain as well as in other organs like liver and kidney. ALDH5A1 operates in mitochondria emphasizing its role in cellular respiration and energy production.
Biological function summary

The enzyme ALDH5A1 plays an important role in the breakdown of gamma-aminobutyric acid (GABA) an important neurotransmitter. It is known to be part of the GABA degradation complex which controls the levels of GABA in the central nervous system. Balance in GABA concentration is necessary for proper neurotransmission and neurophysiological functions. Consequently ALDH5A1 helps in maintaining the inhibitory effects GABA has on neurons.

Pathways

ALDH5A1 is significant in the GABA shunt pathway an alternative mechanism for glutamate and GABA metabolism. The enzyme collaborates closely with GABA transaminase which converts GABA into succinate semialdehyde before ALDH5A1 further processes it into succinate. This pathway supports energy production and the regulation of carbon flow through central metabolism.

ALDH5A1 deficiencies link to disorders like Succinic Semialdehyde Dehydrogenase deficiency (SSADH deficiency) and GABA-transaminase deficiency. These conditions result in the accumulation of gamma-hydroxybutyric acid leading to neurological complications. This enzyme interacts with proteins like GABA transaminase which shows the importance of its proper function in preventing neurologic disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
See full target information ALDH5A1
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Product promise

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