Rabbit Polyclonal ALDH5A1/SSADH antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH5A1 aa 50-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALDH5A1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link P51649
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Suggested antigenretrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Suggested antigenretrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Associated Products
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Target data
Function
Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
Alternative names
SSADH, ALDH5A1, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase
Recommended products
Rabbit Polyclonal ALDH5A1/SSADH antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH5A1 aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALDH5A1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link P51649
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Keep as a concentrated solution.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH5A1 also known as succinate semialdehyde dehydrogenase (SSADH) functions in the cellular metabolism where it converts succinate semialdehyde to succinate in the presence of NAD+. This enzyme has a molecular weight of approximately 56 kDa and is largely found in the brain as well as in other organs like liver and kidney. ALDH5A1 operates in mitochondria emphasizing its role in cellular respiration and energy production.
- Biological function summary
The enzyme ALDH5A1 plays an important role in the breakdown of gamma-aminobutyric acid (GABA) an important neurotransmitter. It is known to be part of the GABA degradation complex which controls the levels of GABA in the central nervous system. Balance in GABA concentration is necessary for proper neurotransmission and neurophysiological functions. Consequently ALDH5A1 helps in maintaining the inhibitory effects GABA has on neurons.
- Pathways
ALDH5A1 is significant in the GABA shunt pathway an alternative mechanism for glutamate and GABA metabolism. The enzyme collaborates closely with GABA transaminase which converts GABA into succinate semialdehyde before ALDH5A1 further processes it into succinate. This pathway supports energy production and the regulation of carbon flow through central metabolism.
- Associated diseases and disorders
ALDH5A1 deficiencies link to disorders like Succinic Semialdehyde Dehydrogenase deficiency (SSADH deficiency) and GABA-transaminase deficiency. These conditions result in the accumulation of gamma-hydroxybutyric acid leading to neurological complications. This enzyme interacts with proteins like GABA transaminase which shows the importance of its proper function in preventing neurologic disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Western blot - Anti-ALDH5A1/SSADH antibody (ab155493)
7.5% SDS PAGE
All lanes: Western blot - Anti-ALDH5A1/SSADH antibody (ab155493) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (ab155493)
Paraffin-embedded mouse liver tissue labeling ALDH5A1/SSADH using ab155493 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
Western blot - Anti-ALDH5A1/SSADH antibody (ab155493)
7.5% SDS PAGE
All lanes: Western blot - Anti-ALDH5A1/SSADH antibody (ab155493) at 1/5000 dilution
All lanes: Mouse brain whole cell lysate at 20 µg
Predicted band size: 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH5A1/SSADH antibody (ab155493)
Immunofluorescent analysis of methanol-fixed A431 cells labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution. Lower panel co-stained with Hoescht 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody (ab155493)
Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution.
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