AB129017

Anti-ALDH5A1/SSADH antibody [EPR7794]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal ALDH5A1/SSADH antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

SSADH, ALDH5A1, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

ab129017 at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

ab129017 at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Mouse kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

All lanes:

Western blot - Anti-ALDH5A1/SSADH antibody [EPR7794] (ab129017) at 1/10000 dilution

Lane 1:

Fetal liver tissue lysate at 10 µg

Lane 2:

A431 lysates at 10 µg

Predicted band size: 57 kDa

Observed band size: 54 kDa

false

OI-RD Scanning - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-ALDH5A1/SSADH antibody [EPR7794] - BSA and Azide free
HRP

HRP Anti-ALDH5A1/SSADH antibody [EPR7794]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7794

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH5A1 also known as succinate semialdehyde dehydrogenase (SSADH) functions in the cellular metabolism where it converts succinate semialdehyde to succinate in the presence of NAD+. This enzyme has a molecular weight of approximately 56 kDa and is largely found in the brain as well as in other organs like liver and kidney. ALDH5A1 operates in mitochondria emphasizing its role in cellular respiration and energy production.

Biological function summary

The enzyme ALDH5A1 plays an important role in the breakdown of gamma-aminobutyric acid (GABA) an important neurotransmitter. It is known to be part of the GABA degradation complex which controls the levels of GABA in the central nervous system. Balance in GABA concentration is necessary for proper neurotransmission and neurophysiological functions. Consequently ALDH5A1 helps in maintaining the inhibitory effects GABA has on neurons.

Pathways

ALDH5A1 is significant in the GABA shunt pathway an alternative mechanism for glutamate and GABA metabolism. The enzyme collaborates closely with GABA transaminase which converts GABA into succinate semialdehyde before ALDH5A1 further processes it into succinate. This pathway supports energy production and the regulation of carbon flow through central metabolism.

ALDH5A1 deficiencies link to disorders like Succinic Semialdehyde Dehydrogenase deficiency (SSADH deficiency) and GABA-transaminase deficiency. These conditions result in the accumulation of gamma-hydroxybutyric acid leading to neurological complications. This enzyme interacts with proteins like GABA transaminase which shows the importance of its proper function in preventing neurologic disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).

See full target information ALDH5A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791277

2024

Functional Characterization of a Spectrum of Genetic Variants in a Family with Succinic Semialdehyde Dehydrogenase Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Miroslava Didiasova,Samuele Cesaro,Simon Feldhoff,Ilaria Bettin,Nana Tiegel,Vera Füssgen,Mariarita Bertoldi,Ritva Tikkanen

International journal of molecular sciences 21: PubMed33203024

2020

Succinic Semialdehyde Dehydrogenase Deficiency: In Vitro and In Silico Characterization of a Novel Pathogenic Missense Variant and Analysis of the Mutational Spectrum of .

Applications

Unspecified application

Species

Unspecified reactive species

Heiko Brennenstuhl,Miroslava Didiasova,Birgit Assmann,Mariarita Bertoldi,Gianluca Molla,Sabine Jung-Klawitter,Oya Kuseyri Hübschmann,Julian Schröter,Thomas Opladen,Ritva Tikkanen

Cell stem cell 26:331-345.e6 PubMed31978364

2020

Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zheng,Hong-Lian Li,Na Tian,Fei Liu,Lu Wang,Yaling Yin,Lupeng Yue,Longyu Ma,You Wan,Jian-Zhi Wang

Journal of experimental & clinical cancer research 37:271 PubMed30404651

2018

GABA, glutamine, glutamate oxidation and succinic semialdehyde dehydrogenase expression in human gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zoltán Hujber,Gergő Horváth,Gábor Petővári,Ildikó Krencz,Titanilla Dankó,Katalin Mészáros,Hajnalka Rajnai,Norbert Szoboszlai,William P J Leenders,András Jeney,László Tretter,Anna Sebestyén

International journal of colorectal disease 28:375-83 PubMed22847606

2012

Glucose transporter 1 (GLUT1) of anaerobic glycolysis as predictive and prognostic values in neoadjuvant chemoradiotherapy and laparoscopic surgery for locally advanced rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Byoung Yong Shim,Ji-Han Jung,Kang-Moon Lee,Hyung-Jin Kim,Sook Hee Hong,Sung Hwan Kim,Der Sheng Sun,Hyeon-Min Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH5A1/SSADH antibody [EPR7794]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

Western blot - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

OI-RD Scanning - Anti-ALDH5A1/SSADH antibody [EPR7794] (AB129017)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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