Rabbit Polyclonal ALDH7A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH7A1 aa 350 to C-terminus.
View Alternative Names
ATQ1, ALDH7A1, Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase
- WB
Supplier Data
Western blot - Anti-ALDH7A1 antibody (AB236663)
All lanes:
Western blot - Anti-ALDH7A1 antibody (ab236663) at 1/500 dilution
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2:
HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4:
SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5:
Rat liver lysate
Lane 6:
Rat kidney lysate
Lane 7:
Mouse liver lysate
Lane 8:
Mouse kidney lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)
Paraffin-embedded human kidney tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)
Paraffin-embedded human liver tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IP
Supplier Data
Immunoprecipitation - Anti-ALDH7A1 antibody (AB236663)
ALDH7A1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236663.
Lane 1 : Rabbit control IgG instead of ab236663 in HeLa whole cell lysate.
Lane 2 : 6 μg ab236663 IP in HeLa whole cell lysate.
Lane 3 : HeLa whole cell lysate 20 μg (Input).
All lanes:
Immunoprecipitation - Anti-ALDH7A1 antibody (ab236663)
Predicted band size: 58 kDa
false
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (AB236663)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ALDH7A1 using ab236663 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.
Pathways
Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.
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