Rabbit Polyclonal ALDH7A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH7A1 aa 350 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALDH7A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49419
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.
Alternative names
ATQ1, ALDH7A1, ATQ1, Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase
Recommended products
Rabbit Polyclonal ALDH7A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH7A1 aa 350 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALDH7A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49419
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALDH7A1 also known as antiquitin is an enzyme that functions in the cellular metabolism processes specifically catalyzing the oxidation of aldehydes. It has a molecular mass of about 54 kDa and can be found expressed in various tissues including liver and brain. This enzyme is part of the aldehyde dehydrogenase family which plays significant roles in detoxifying aldehydes generated by alcohol metabolism and other metabolic activities.
- Biological function summary
The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.
- Pathways
Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.
- Associated diseases and disorders
Defects in ALDH7A1 can lead to pyridoxine-dependent epilepsy a rare genetic disorder caused by the accumulation of toxic metabolites due to impaired enzyme function. The disorder influences neurological development and can lead to seizures without treatment. ALDH7A1 dysfunction is also linked with aldehyde dehydrogenase liver deficiency where similarities with ALDH2 — another enzyme from the same family — can influence symptoms like alcohol sensitivity and metabolic responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-ALDH7A1 antibody (ab236663)
All lanes: Western blot - Anti-ALDH7A1 antibody (ab236663) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5: Rat liver lysate
Lane 6: Rat kidney lysate
Lane 7: Mouse liver lysate
Lane 8: Mouse kidney lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab236663)
Paraffin-embedded human kidney tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab236663)
Paraffin-embedded human liver tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitation - Anti-ALDH7A1 antibody (ab236663)
ALDH7A1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236663.
Lane 1: Rabbit control IgG instead of ab236663 in HeLa whole cell lysate.
Lane 2: 6 μg ab236663 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 μg (Input).All lanes: Immunoprecipitation - Anti-ALDH7A1 antibody (ab236663)
Predicted band size: 58 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (ab236663)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ALDH7A1 using ab236663 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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